About: Comparison of DNA extraction methods in terms of yield, purity, long-term storage and downstream manipulation with brewer's yeast chromosomal DNA

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1094/ASBCJ-2014-0110-01
Description	Five methods of DNA isolation (a „classical“ phenol/chloroform extraction and four commercially available kits) were compared in terms of the yield, purity, integrity and stability of extracted DNA from both laboratory and industrial yeast strains. DNA isolation with commercial kits employing silica based adsorption collumns mostly gave lower yields of DNA, integrity of DNA soon declines and after six months of storage under the manufacturer's instructions the DNA was almost fully degraded. Isolation of DNA by using bead breaking of cells resulted in very low yield and low purity of the DNA also. The use of a commercial kit without silica collumns and phenol/chloroform treatment as well as the traditional phenol/chloroform extraction were certainly more time-consuming, but the extracted DNA was of high purity and concentration, with minimal damage, stable over months and usable for subsequent molecular analyses (cloning, PCR, RFLP, DNA sequencing). Five methods of DNA isolation (a „classical“ phenol/chloroform extraction and four commercially available kits) were compared in terms of the yield, purity, integrity and stability of extracted DNA from both laboratory and industrial yeast strains. DNA isolation with commercial kits employing silica based adsorption collumns mostly gave lower yields of DNA, integrity of DNA soon declines and after six months of storage under the manufacturer's instructions the DNA was almost fully degraded. Isolation of DNA by using bead breaking of cells resulted in very low yield and low purity of the DNA also. The use of a commercial kit without silica collumns and phenol/chloroform treatment as well as the traditional phenol/chloroform extraction were certainly more time-consuming, but the extracted DNA was of high purity and concentration, with minimal damage, stable over months and usable for subsequent molecular analyses (cloning, PCR, RFLP, DNA sequencing). (en)
Title	Comparison of DNA extraction methods in terms of yield, purity, long-term storage and downstream manipulation with brewer's yeast chromosomal DNA Comparison of DNA extraction methods in terms of yield, purity, long-term storage and downstream manipulation with brewer's yeast chromosomal DNA (en)
skos:prefLabel	Comparison of DNA extraction methods in terms of yield, purity, long-term storage and downstream manipulation with brewer's yeast chromosomal DNA Comparison of DNA extraction methods in terms of yield, purity, long-term storage and downstream manipulation with brewer's yeast chromosomal DNA (en)
skos:notation	RIV/00216224:14310/14:00075066!RIV15-MSM-14310___
http://linked.open...avai/riv/aktivita	I S
http://linked.open...avai/riv/aktivity	I, S
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Kopecká, Jana Němec, Miroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	8059
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/14:00075066
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	brewer's yeast; isolation of DNA; phenol/chloroform extraction; purification (en)
http://linked.open.../riv/klicoveSlovo	isolation of DNA purification brewer's yeast phenol/chloroform extraction
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[D2E9E8DADBFC]
http://linked.open...i/riv/nazevZdroje	The Journal of the American Society of Brewing Chemists
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	72
http://linked.open...iv/tvurceVysledku	Felsberg, Jürgen Jelínková, Markéta Kopecká, Jana Němec, Miroslav Matoulková, Dagmar
http://linked.open...ain/vavai/riv/wos	000332594500001
issn	0361-0470
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1094/ASBCJ-2014-0110-01
http://localhost/t...ganizacniJednotka	14310

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