About: Computer-Assisted Engineering of Synthetic Pathway for Biodegradation of Toxic Persistent Pollutant.

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Computer-Assisted Engineering of Synthetic Pathway for Biodegradation of Toxic Persistent Pollutant. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Anthropogenic halogenated compounds were unknown to Nature until the industrial revolution, and microorganisms have not had sufficient time to evolve enzymes for their degradation. The lack of efficient enzymes and natural pathways can be addressed through a combination of protein and metabolic engineering. We have assembled a synthetic route for conversion of the highly toxic and recalcitrant 1,2,3-trichloropropane to glycerol in Escherichia coli, and used it for a systematic study of pathway bottlenecks. Optimal ratios of enzymes for the maximal production of glycerol, and minimal toxicity of metabolites were predicted using a mathematical model. The strains containing the expected optimal ratios of enzymes were constructed and characterized for their viability and degradation efficiency. Excellent agreement between predicted and experimental data was observed. Anthropogenic halogenated compounds were unknown to Nature until the industrial revolution, and microorganisms have not had sufficient time to evolve enzymes for their degradation. The lack of efficient enzymes and natural pathways can be addressed through a combination of protein and metabolic engineering. We have assembled a synthetic route for conversion of the highly toxic and recalcitrant 1,2,3-trichloropropane to glycerol in Escherichia coli, and used it for a systematic study of pathway bottlenecks. Optimal ratios of enzymes for the maximal production of glycerol, and minimal toxicity of metabolites were predicted using a mathematical model. The strains containing the expected optimal ratios of enzymes were constructed and characterized for their viability and degradation efficiency. Excellent agreement between predicted and experimental data was observed. (en)
Title	Computer-Assisted Engineering of Synthetic Pathway for Biodegradation of Toxic Persistent Pollutant. Computer-Assisted Engineering of Synthetic Pathway for Biodegradation of Toxic Persistent Pollutant. (en)
skos:prefLabel	Computer-Assisted Engineering of Synthetic Pathway for Biodegradation of Toxic Persistent Pollutant. Computer-Assisted Engineering of Synthetic Pathway for Biodegradation of Toxic Persistent Pollutant. (en)
skos:notation	RIV/00216224:14310/14:00074211!RIV15-MSM-14310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED0001/01/01), P(EE2.3.30.0037), P(GAP503/12/0572)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Prokop, Zbyněk Damborský, Jiří Dvořák, Pavel Brezovský, Jan Bendl, Jaroslav Kurumbang, Nagendra Prasad
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	8507
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/14:00074211
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	activity; enantioselectivity; kinetic modeling; protein and metabolic engineering; synthetic pathway; toxicity (en)
http://linked.open.../riv/klicoveSlovo	enantioselectivity toxicity activity kinetic modeling protein and metabolic engineering synthetic pathway
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[659D51423945]
http://linked.open...i/riv/nazevZdroje	ACS Synthetic Biology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Employment of Best Young Scientists for International Cooperation Empowerment Construction of a synthetic metabolic pathway for degradation of important environmental pollutant using protein and metabolic engineering CETOCOEN
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	3
http://linked.open...iv/tvurceVysledku	Brezovský, Jan Damborský, Jiří Dvořák, Pavel Prokop, Zbyněk Bendl, Jaroslav Kurumbang, Nagendra Prasad
http://linked.open...ain/vavai/riv/wos	000333476900009
issn	2161-5063
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1021/sb400147n
http://localhost/t...ganizacniJednotka	14310

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software