About: Dynamics and Hydration Explain Failed Functional Transformation in Dehalogenase Design.

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About: Dynamics and Hydration Explain Failed Functional Transformation in Dehalogenase Design. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We emphasize the importance of dynamics and hydration for enzymatic catalysis and protein design by transplanting the active site from a haloalkane dehalogenase with high enantioselectivity to nonselective dehalogenase. Protein crystallography confirms that the active site geometry of the redesigned dehalogenase matches that of the target, but its enantioselectivity remains low. Time-dependent fluorescence shifts and computer simulations revealed that dynamics and hydration at the tunnel mouth differ substantially between the redesigned and target dehalogenase. We emphasize the importance of dynamics and hydration for enzymatic catalysis and protein design by transplanting the active site from a haloalkane dehalogenase with high enantioselectivity to nonselective dehalogenase. Protein crystallography confirms that the active site geometry of the redesigned dehalogenase matches that of the target, but its enantioselectivity remains low. Time-dependent fluorescence shifts and computer simulations revealed that dynamics and hydration at the tunnel mouth differ substantially between the redesigned and target dehalogenase. (en)
Title	Dynamics and Hydration Explain Failed Functional Transformation in Dehalogenase Design. Dynamics and Hydration Explain Failed Functional Transformation in Dehalogenase Design. (en)
skos:prefLabel	Dynamics and Hydration Explain Failed Functional Transformation in Dehalogenase Design. Dynamics and Hydration Explain Failed Functional Transformation in Dehalogenase Design. (en)
skos:notation	RIV/00216224:14310/14:00074209!RIV15-MSM-14310___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED1.100/02/0123), P(EE2.3.30.0037), P(GAP503/12/0572), P(GBP208/12/G016), P(LO1214)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Koudeláková, Táňa Prokop, Zbyněk Fořtová, Andrea Štěpánková, Veronika Damborský, Jiří Brezovský, Jan Chaloupková, Radka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	12724
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/14:00074209
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	haloalkane dehalogenase (en)
http://linked.open.../riv/klicoveSlovo	haloalkane dehalogenase
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[DF36122EBB67]
http://linked.open...i/riv/nazevZdroje	Nature Chemical Biology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	12 (xsd:int)
http://linked.open...vavai/riv/projekt	Employment of Best Young Scientists for International Cooperation Empowerment Controlling structure and function of biomolecules at the molecular scale: theory meets experiment Construction of a synthetic metabolic pathway for degradation of important environmental pollutant using protein and metabolic engineering Centrum pro výzkum toxických látek v prostředí St. Anne´s University Hospital Brno - International Clinical Research Center (FNUSA-ICRC)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	10
http://linked.open...iv/tvurceVysledku	Brezovský, Jan Chaloupková, Radka Damborský, Jiří Fořtová, Andrea Koudeláková, Táňa Prokop, Zbyněk Štěpánková, Veronika Kuta Smatanova, I. Hof, M. Sykora, J. Lahoda, M. Chernovets, T.
http://linked.open...ain/vavai/riv/wos	000336238200008
issn	1552-4450
number of pages	3 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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