About: Microscopic Monitoring Provides Information on Structure and Properties During Biocatalyst Immobilisation.

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About: Microscopic Monitoring Provides Information on Structure and Properties During Biocatalyst Immobilisation. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Enzymes have a wide range of applications in different industries owing to their high specificity and efficiency. Immobilisation is often used to improve biocatalyst properties, operational stability and reusability. However, changes in the structure of biocatalysts during immobilisation and under process conditions are still largely uncertain. Here, three microscopy techniques – bright-field, confocal and electron microscopy – were applied to determine the distribution and structure of an immobilised biocatalyst. Free enzyme (haloalkane dehalogenase), cross-linked enzyme aggregates (CLEAs) and CLEAs entrapped in polyvinyl alcohol lenses (lentikats) were used as model systems. Electron microscopy revealed that sonicated CLEAs underwent morphological changes that strongly correlated with increased catalytic activity compared to less structured, non-treated CLEAs. Confocal microscopy confirmed that loading of the biocatalyst was not the only factor affecting the catalytic activity of the lentikats. Enzymes have a wide range of applications in different industries owing to their high specificity and efficiency. Immobilisation is often used to improve biocatalyst properties, operational stability and reusability. However, changes in the structure of biocatalysts during immobilisation and under process conditions are still largely uncertain. Here, three microscopy techniques – bright-field, confocal and electron microscopy – were applied to determine the distribution and structure of an immobilised biocatalyst. Free enzyme (haloalkane dehalogenase), cross-linked enzyme aggregates (CLEAs) and CLEAs entrapped in polyvinyl alcohol lenses (lentikats) were used as model systems. Electron microscopy revealed that sonicated CLEAs underwent morphological changes that strongly correlated with increased catalytic activity compared to less structured, non-treated CLEAs. Confocal microscopy confirmed that loading of the biocatalyst was not the only factor affecting the catalytic activity of the lentikats. (en)
Title	Microscopic Monitoring Provides Information on Structure and Properties During Biocatalyst Immobilisation. Microscopic Monitoring Provides Information on Structure and Properties During Biocatalyst Immobilisation. (en)
skos:prefLabel	Microscopic Monitoring Provides Information on Structure and Properties During Biocatalyst Immobilisation. Microscopic Monitoring Provides Information on Structure and Properties During Biocatalyst Immobilisation. (en)
skos:notation	RIV/00216224:14310/14:00074208!RIV15-MSM-14310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.100/02/0123), P(EE2.3.20.0183), P(GAP207/12/0775), P(LO1214)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Prokop, Zbyněk Hampl, Aleš Ilkovics, Ladislav Damborský, Jiří Bidmanová, Šárka Jaroš, Josef
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	29223
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/14:00074208
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Cross-linked enzyme aggregates; Immobilization; Microscopy; Polyvinyl alcohol; Structure (en)
http://linked.open.../riv/klicoveSlovo	Cross-linked enzyme aggregates Immobilization Polyvinyl alcohol Structure Microscopy
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[956B46AAC01D]
http://linked.open...i/riv/nazevZdroje	Biotechnology Journal
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Strukturně-funkční vztahy haloalkan dehalogenas Center of Experimental Biomedicine Centrum pro výzkum toxických látek v prostředí St. Anne´s University Hospital Brno - International Clinical Research Center (FNUSA-ICRC)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	9
http://linked.open...iv/tvurceVysledku	Bidmanová, Šárka Damborský, Jiří Hampl, Aleš Hrdličková, Eva Prokop, Zbyněk Jaroš, Josef Ilkovics, Ladislav
http://linked.open...ain/vavai/riv/wos	000337700000015
issn	1860-6768
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/biot.201300049
http://localhost/t...ganizacniJednotka	14310

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