About: Fructose 1-Phosphate is the One and Only Physiological Effector of the Cra (FruR) regulator of Pseudomonas putida.

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About: Fructose 1-Phosphate is the One and Only Physiological Effector of the Cra (FruR) regulator of Pseudomonas putida. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Fructose-1-phosphate (F1P) is the preferred effector of the catabolite repressor/activator (Cra) protein of the soil bacterium Pseudomonas putida but its ability to bind other metabolic intermediates in vivo is unclear. The Cra protein of this microorganism (CraPP) was submitted to mobility shift assays with target DNA sequences (the PfruB promoter) and candidate effectors fructose-1,6-biphosphate (FBP), glucose 6-phosphate (G6P), and fructose-6-phosphate (F6P). 1mM F1P was sufficient to release most of the Cra protein from its operators but more than 10mM of FBP or G6P was required to free the same complex. However, isothermal titration microcalorimetry failed to expose any specific interaction between CraPP and FBP or G6P. To solve this paradox, transcriptional activity of a PfruB-lacZ fusion was measured in wild-type and fruB cells growing on substrates that change the intracellular concentrations of F1P and FBP. Fructose-1-phosphate (F1P) is the preferred effector of the catabolite repressor/activator (Cra) protein of the soil bacterium Pseudomonas putida but its ability to bind other metabolic intermediates in vivo is unclear. The Cra protein of this microorganism (CraPP) was submitted to mobility shift assays with target DNA sequences (the PfruB promoter) and candidate effectors fructose-1,6-biphosphate (FBP), glucose 6-phosphate (G6P), and fructose-6-phosphate (F6P). 1mM F1P was sufficient to release most of the Cra protein from its operators but more than 10mM of FBP or G6P was required to free the same complex. However, isothermal titration microcalorimetry failed to expose any specific interaction between CraPP and FBP or G6P. To solve this paradox, transcriptional activity of a PfruB-lacZ fusion was measured in wild-type and fruB cells growing on substrates that change the intracellular concentrations of F1P and FBP. (en)
Title	Fructose 1-Phosphate is the One and Only Physiological Effector of the Cra (FruR) regulator of Pseudomonas putida. Fructose 1-Phosphate is the One and Only Physiological Effector of the Cra (FruR) regulator of Pseudomonas putida. (en)
skos:prefLabel	Fructose 1-Phosphate is the One and Only Physiological Effector of the Cra (FruR) regulator of Pseudomonas putida. Fructose 1-Phosphate is the One and Only Physiological Effector of the Cra (FruR) regulator of Pseudomonas putida. (en)
skos:notation	RIV/00216224:14310/14:00074207!RIV15-MSM-14310___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED0001/01/01), P(EE2.3.30.0037), P(GAP503/12/0572)
http://linked.open...iv/cisloPeriodika	2014
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Damborský, Jiří Brezovský, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	17599
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/14:00074207
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Cra; FruR; Pseudomonas putida; Fructose 1-phosphate; Fructose operon (en)
http://linked.open.../riv/klicoveSlovo	Cra FruR Fructose 1-phosphate Fructose operon Pseudomonas putida
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[E4825EA3B338]
http://linked.open...i/riv/nazevZdroje	FEBS Open Bio
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Employment of Best Young Scientists for International Cooperation Empowerment Construction of a synthetic metabolic pathway for degradation of important environmental pollutant using protein and metabolic engineering CETOCOEN
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	4
http://linked.open...iv/tvurceVysledku	Brezovský, Jan Damborský, Jiří Chavarria, M. Durante-Rodriguez, G. Krell, T. Santiago, C. de Lorenzo, V.
http://linked.open...ain/vavai/riv/wos	000346278200048
issn	2211-5463
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.fob.2014.03.013
http://localhost/t...ganizacniJednotka	14310

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