About: Structural and Functional Analysis of a Novel Haloalkane Dehalogenase with Two Halide-Binding Sites.

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About: Structural and Functional Analysis of a Novel Haloalkane Dehalogenase with Two Halide-Binding Sites. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Crystal structure of novel haloalkane dehalogenase DbeA revealed the presence of two chloride ions buried in the protein interior. The first halide-binding site is involved in substrate binding and is present in all structurally characterized haloalkane dehalogenases. The second halide-binding site is unique to DbeA. To elucidate the role of the second halide-binding site in enzyme functionality, a two-point mutant lacking this site was constructed and characterized. These substitutions resulted in a shift of substrate-specificity class and were accompanied by decrease of enzyme activity, stability and elimination of the substrate inhibition. The changes in enzyme catalytic activity were attributed to deceleration of the rate-limiting hydrolytic step, mediated by lower basicity of the catalytic histidine. Crystal structure of novel haloalkane dehalogenase DbeA revealed the presence of two chloride ions buried in the protein interior. The first halide-binding site is involved in substrate binding and is present in all structurally characterized haloalkane dehalogenases. The second halide-binding site is unique to DbeA. To elucidate the role of the second halide-binding site in enzyme functionality, a two-point mutant lacking this site was constructed and characterized. These substitutions resulted in a shift of substrate-specificity class and were accompanied by decrease of enzyme activity, stability and elimination of the substrate inhibition. The changes in enzyme catalytic activity were attributed to deceleration of the rate-limiting hydrolytic step, mediated by lower basicity of the catalytic histidine. (en)
Title	Structural and Functional Analysis of a Novel Haloalkane Dehalogenase with Two Halide-Binding Sites. Structural and Functional Analysis of a Novel Haloalkane Dehalogenase with Two Halide-Binding Sites. (en)
skos:prefLabel	Structural and Functional Analysis of a Novel Haloalkane Dehalogenase with Two Halide-Binding Sites. Structural and Functional Analysis of a Novel Haloalkane Dehalogenase with Two Halide-Binding Sites. (en)
skos:notation	RIV/00216224:14310/14:00074206!RIV15-MSM-14310___
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(GAP207/12/0775), P(LO1214), S
http://linked.open...iv/cisloPeriodika	July
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Daniel, Lukáš Koudeláková, Táňa Prokop, Zbyněk Damborský, Jiří Brezovský, Jan Chaloupková, Radka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	47818
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/14:00074206
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	haloalkane dehalogenase DbeA from Bradyrhizobium elkanii USDA94 (en)
http://linked.open.../riv/klicoveSlovo	haloalkane dehalogenase DbeA from Bradyrhizobium elkanii USDA94
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[42A70CDB78D1]
http://linked.open...i/riv/nazevZdroje	Acta Crystallographica D
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	13 (xsd:int)
http://linked.open...vavai/riv/projekt	Strukturně-funkční vztahy haloalkan dehalogenas Centrum pro výzkum toxických látek v prostředí
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	70
http://linked.open...iv/tvurceVysledku	Brezovský, Jan Chaloupková, Radka Damborský, Jiří Koudeláková, Táňa Prokop, Zbyněk Nagata, Y. Prudnikova, T. Sato, Y. Kuta Smatanova, I. Kuty, M. Rezacova, P. Daniel, Lukáš Ikeda-Ohtsubo, W.
http://linked.open...ain/vavai/riv/wos	000338917000009
issn	0907-4449
number of pages	14 (xsd:int)
http://bibframe.org/vocab/doi	10.1107/S1399004714009018
http://localhost/t...ganizacniJednotka	14310

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