About: Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization.

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About: Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Multi-enzyme processes represent an important area of biocatalysis. Their efficiency can be enhanced by optimization of biocatalysts’ stoichiometry. Here we present a workflow for maximizing the efficiency of a three-enzyme system catalysing a five-step chemical conversion. Kinetic models of pathways featuring either wild-type or engineered enzymes were built and the enzyme stoichiometry of each pathway was optimized. Mathematical modelling and one-pot multi-enzyme laboratory experiments provided detailed insights into pathway dynamics, enabled the selection of suitable engineered enzyme and afforded high efficiency while minimizing biocatalyst loadings. The optimizing of stoichiometry in a pathway with engineered enzyme reduced the total biocatalyst load by an impressive 56 %. Our new workflow represents a broadly applicable strategy for optimizing multi-enzyme processes. Multi-enzyme processes represent an important area of biocatalysis. Their efficiency can be enhanced by optimization of biocatalysts’ stoichiometry. Here we present a workflow for maximizing the efficiency of a three-enzyme system catalysing a five-step chemical conversion. Kinetic models of pathways featuring either wild-type or engineered enzymes were built and the enzyme stoichiometry of each pathway was optimized. Mathematical modelling and one-pot multi-enzyme laboratory experiments provided detailed insights into pathway dynamics, enabled the selection of suitable engineered enzyme and afforded high efficiency while minimizing biocatalyst loadings. The optimizing of stoichiometry in a pathway with engineered enzyme reduced the total biocatalyst load by an impressive 56 %. Our new workflow represents a broadly applicable strategy for optimizing multi-enzyme processes. (en)
Title	Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization. Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization. (en)
skos:prefLabel	Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization. Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization. (en)
skos:notation	RIV/00216224:14310/14:00074203!RIV15-MSM-14310___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED1.100/02/0123), P(EE2.3.30.0037), P(GAP503/12/0572)
http://linked.open...iv/cisloPeriodika	13
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Prokop, Zbyněk Damborský, Jiří Dvořák, Pavel Brezovský, Jan Bendl, Jaroslav Kurumbang, Nagendra Prasad
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	27707
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/14:00074203
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	biocatalysis; engineered enzymes (en)
http://linked.open.../riv/klicoveSlovo	biocatalysis engineered enzymes
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[32E632E67008]
http://linked.open...i/riv/nazevZdroje	ChemBioChem
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Employment of Best Young Scientists for International Cooperation Empowerment Construction of a synthetic metabolic pathway for degradation of important environmental pollutant using protein and metabolic engineering St. Anne´s University Hospital Brno - International Clinical Research Center (FNUSA-ICRC)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	15
http://linked.open...iv/tvurceVysledku	Brezovský, Jan Damborský, Jiří Dvořák, Pavel Prokop, Zbyněk Bendl, Jaroslav Kurumbang, Nagendra Prasad
http://linked.open...ain/vavai/riv/wos	000341586100007
issn	1439-4227
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/cbic.201402265
http://localhost/t...ganizacniJednotka	14310

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