About: A Pseudomonas putida Strain Genetically Engineered for 1,2,3-Trichloropropane Bioremediation.

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About: A Pseudomonas putida Strain Genetically Engineered for 1,2,3-Trichloropropane Bioremediation. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	1,2,3-Trichloropropane (TCP) is a toxic compound that is recalcitrant to biodegradation in the environment and attempts to isolate TCP-degrading organisms using enrichment cultivation have failed. A potential biodegradation pathway starts with hydrolytic dehalogenation to 2,3 dichloro-1-propanol (DCP), followed by oxidative metabolism. To obtain a practically applicable TCP-degrading organism, we introduced an engineered haloalkane dehalogenase with improved TCP degradation activity into the DCP-degrading bacterium Pseudomonas putida MC4. For this, the dehalogenase gene (dhaA31) was cloned behind the constitutive dhlA promoter and introduced into the genome of strain MC4 using a transposon delivery system. The transposon-located antibiotic resistance marker was subsequently removed using a resolvase step. Growth of the resulting engineered bacterium P. putida MC4-5222 on TCP was indeed observed, and all organic chlorine was released as chloride. 1,2,3-Trichloropropane (TCP) is a toxic compound that is recalcitrant to biodegradation in the environment and attempts to isolate TCP-degrading organisms using enrichment cultivation have failed. A potential biodegradation pathway starts with hydrolytic dehalogenation to 2,3 dichloro-1-propanol (DCP), followed by oxidative metabolism. To obtain a practically applicable TCP-degrading organism, we introduced an engineered haloalkane dehalogenase with improved TCP degradation activity into the DCP-degrading bacterium Pseudomonas putida MC4. For this, the dehalogenase gene (dhaA31) was cloned behind the constitutive dhlA promoter and introduced into the genome of strain MC4 using a transposon delivery system. The transposon-located antibiotic resistance marker was subsequently removed using a resolvase step. Growth of the resulting engineered bacterium P. putida MC4-5222 on TCP was indeed observed, and all organic chlorine was released as chloride. (en)
Title	A Pseudomonas putida Strain Genetically Engineered for 1,2,3-Trichloropropane Bioremediation. A Pseudomonas putida Strain Genetically Engineered for 1,2,3-Trichloropropane Bioremediation. (en)
skos:prefLabel	A Pseudomonas putida Strain Genetically Engineered for 1,2,3-Trichloropropane Bioremediation. A Pseudomonas putida Strain Genetically Engineered for 1,2,3-Trichloropropane Bioremediation. (en)
skos:notation	RIV/00216224:14310/14:00074202!RIV15-MSM-14310___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP503/12/0572)
http://linked.open...iv/cisloPeriodika	17
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Pavlová, Martina Damborský, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	1075
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/14:00074202
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	1; 2; 3-Trichloropropane; biodegradation; Pseudomonas putida MC4 (en)
http://linked.open.../riv/klicoveSlovo	1 2 biodegradation 3-Trichloropropane Pseudomonas putida MC4
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[EE9505561FA6]
http://linked.open...i/riv/nazevZdroje	Applied and Environmental Microbiology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Construction of a synthetic metabolic pathway for degradation of important environmental pollutant using protein and metabolic engineering
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	80
http://linked.open...iv/tvurceVysledku	Damborský, Jiří Pavlová, Martina Janssen, D. B. Arif, M. I. Ghufrana, S. Postema, C. P.
http://linked.open...ain/vavai/riv/wos	000341486500032
issn	0099-2240
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1128/AEM.01620-14
http://localhost/t...ganizacniJednotka	14310

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