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  • Freeze-fracture technique is method for visualisation membrane surfaces of cell organelles. This method used cryo-fixation for stabilization a sample. Sample is frozen in nitrogen and cut in the chamber under a vacuum and low temperature. Glycerol is used as a cryoprotectant for observation fine and smooth cellular structures. Although, cryoprotectants serve for the substitution water and elimination ice crystals production, they could affect a form of fracture through biological membranes and could affect interpretation of morphological structures.
  • Freeze-fracture technique is method for visualisation membrane surfaces of cell organelles. This method used cryo-fixation for stabilization a sample. Sample is frozen in nitrogen and cut in the chamber under a vacuum and low temperature. Glycerol is used as a cryoprotectant for observation fine and smooth cellular structures. Although, cryoprotectants serve for the substitution water and elimination ice crystals production, they could affect a form of fracture through biological membranes and could affect interpretation of morphological structures. (en)
Title
  • Freezer-fracture technique and artefacts caused by processing conditions.
  • Freezer-fracture technique and artefacts caused by processing conditions. (en)
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  • Freezer-fracture technique and artefacts caused by processing conditions.
  • Freezer-fracture technique and artefacts caused by processing conditions. (en)
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  • RIV/00216224:14310/14:00073934!RIV15-GA0-14310___
http://linked.open...avai/riv/aktivita
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  • P(GAP506/12/1258)
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  • RIV/00216224:14310/14:00073934
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  • freeze-fracture technique; artefacts; cryoprotectant; fragmentation; morphological structures (en)
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  • [AE1964BF42AC]
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  • Hodová, Iveta
  • Valigurová, Andrea
  • Melicherová, Janka
  • Vaškovicová, Naděžda
  • Krzyžánek, Vladislav
http://localhost/t...ganizacniJednotka
  • 14310
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