About: Infection of honeybee larvae by entomopathogenic nematodes

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About: Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis and Steinernema are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteria of the genera Photorhabdus or Xenorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves and for expanding nematodes. We used EPNs infection to study immunity of Drosophila melanogaster and here we show for the first time that also honeybee larvae are suitable hosts for nematobacterial complex. We used the tripartite model (honeybee, nematodes, bacteria) to develop standard procedure for testing honeybees' immune response. We optimised the infection for EPN Heterorhabditis bacteriophora and Steinernema feltiae; both species cause typical coloration of cadavers (red or yellow, respectively), multiply in honeybee larvae and shows similar mortality with LD50 approx. Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis and Steinernema are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteria of the genera Photorhabdus or Xenorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves and for expanding nematodes. We used EPNs infection to study immunity of Drosophila melanogaster and here we show for the first time that also honeybee larvae are suitable hosts for nematobacterial complex. We used the tripartite model (honeybee, nematodes, bacteria) to develop standard procedure for testing honeybees' immune response. We optimised the infection for EPN Heterorhabditis bacteriophora and Steinernema feltiae; both species cause typical coloration of cadavers (red or yellow, respectively), multiply in honeybee larvae and shows similar mortality with LD50 approx. (en)
Title	Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity (en)
skos:prefLabel	Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity (en)
skos:notation	RIV/00216224:14310/14:00073256!RIV15-MZE-14310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QJ1210047)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Vojtek, Libor Hyršl, Pavel Dobeš, Pavel
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	21270
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/14:00073256
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	entomopathogenic nematodes; honeaybee; Photorhabdus luminescens; Heterorhabditis bacteriophora (en)
http://linked.open.../riv/klicoveSlovo	honeaybee Heterorhabditis bacteriophora Photorhabdus luminescens entomopathogenic nematodes
http://linked.open...ontrolniKodProRIV	[8A2089D4B824]
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Research and development of novel agents for honey bee health improvement
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Dobeš, Pavel Hyršl, Pavel Vojtek, Libor Berka, Jakub
http://localhost/t...ganizacniJednotka	14310

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