About: Organic Co-solvents Affect Activity, Stability and Enantioselectivity of Haloalkane Dehalogenases

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Haloalkane dehalogenases are microbial enzymes with a wide range of biotechnological applications. The use of organic co-solvents to solubilize their hydrophobic substrates is often necessary. In order to choose the most compatible co-solvent, the effects of fourteen co-solvents on activity, stability and enantioselectivity of three model enzymes, DbjA, DhaA and LinB, were evaluated. All co-solvents caused at high concentration loss of activity and conformational changes. The highest inactivation was induced by tetrahydrofuran, while more hydrophilic co-solvents, such as ethylene glycol and dimethyl sulfoxide, were more tolerated. The effects of co-solvents at low concentration were different for each enzyme-solvent pair. The increase in DbjA activity was induced by the majority of organic co-solvents tested, while activities of DhaA and LinB decreased at comparable concentrations of the same co-solvent. Moreover, high increase of DbjA enantioselectivity was observed. Haloalkane dehalogenases are microbial enzymes with a wide range of biotechnological applications. The use of organic co-solvents to solubilize their hydrophobic substrates is often necessary. In order to choose the most compatible co-solvent, the effects of fourteen co-solvents on activity, stability and enantioselectivity of three model enzymes, DbjA, DhaA and LinB, were evaluated. All co-solvents caused at high concentration loss of activity and conformational changes. The highest inactivation was induced by tetrahydrofuran, while more hydrophilic co-solvents, such as ethylene glycol and dimethyl sulfoxide, were more tolerated. The effects of co-solvents at low concentration were different for each enzyme-solvent pair. The increase in DbjA activity was induced by the majority of organic co-solvents tested, while activities of DhaA and LinB decreased at comparable concentrations of the same co-solvent. Moreover, high increase of DbjA enantioselectivity was observed. (en)
Title	Organic Co-solvents Affect Activity, Stability and Enantioselectivity of Haloalkane Dehalogenases Organic Co-solvents Affect Activity, Stability and Enantioselectivity of Haloalkane Dehalogenases (en)
skos:prefLabel	Organic Co-solvents Affect Activity, Stability and Enantioselectivity of Haloalkane Dehalogenases Organic Co-solvents Affect Activity, Stability and Enantioselectivity of Haloalkane Dehalogenases (en)
skos:notation	RIV/00216224:14310/13:00065823!RIV14-MSM-14310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED0001/01/01), P(GA203/08/0114), P(GAP207/12/0775), P(GAP503/12/0572), P(IAA401630901)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Štěpánková, Veronika Damborský, Jiří Chaloupková, Radka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	94584
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/13:00065823
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Haloalkane Dehalogenases (en)
http://linked.open.../riv/klicoveSlovo	Haloalkane Dehalogenases
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[3340C457C92D]
http://linked.open...i/riv/nazevZdroje	Biotechnology Journal
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Strukturně-funkční vztahy haloalkan dehalogenas Construction of a synthetic metabolic pathway for degradation of important environmental pollutant using protein and metabolic engineering CETOCOEN Specific ion effects for proteins in solutions and related biologically relevant systems. Evolution of substrate specificity in enzymes acting on xenobiotic compounds
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	8
http://linked.open...iv/tvurceVysledku	Chaloupková, Radka Damborský, Jiří Štěpánková, Veronika
http://linked.open...ain/vavai/riv/wos	000320031900013
issn	1860-6768
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/biot.201200378
http://localhost/t...ganizacniJednotka	14310

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