About: The Effect of a Unique Halide-Stabilising Residue on the Catalytic Properties of Haloalkane Dehalogenase DatA from Agrobacterium tumefaciens C58

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About: The Effect of a Unique Halide-Stabilising Residue on the Catalytic Properties of Haloalkane Dehalogenase DatA from Agrobacterium tumefaciens C58 Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Haloalkane dehalogenases catalyse the hydrolysis of carbon-halogen bonds in various chlorinated, brominated and iodinated compounds. These enzymes have a conserved pair of halide-stabilising residues that are important in substrate binding and stabilisation of the transition state and the halide ion product via hydrogen bonding. In all previously known haloalkane dehalogenase, these residues are either a pair of tryptophans or a tryptophan-asparagine pair. The newly isolated haloalkane dehalogenase DatA from Agrobacterium tumefaciens C58 possesses a unique halide-stabilising tyrosine residue, Y109, in place of the conventional tryptophan. A variant of DatA with the Y109W mutation was created and the effects of this mutation on the enzyme’s structure and catalytic properties were studied using spectroscopy and pre-steady-state kinetic experiments. Haloalkane dehalogenases catalyse the hydrolysis of carbon-halogen bonds in various chlorinated, brominated and iodinated compounds. These enzymes have a conserved pair of halide-stabilising residues that are important in substrate binding and stabilisation of the transition state and the halide ion product via hydrogen bonding. In all previously known haloalkane dehalogenase, these residues are either a pair of tryptophans or a tryptophan-asparagine pair. The newly isolated haloalkane dehalogenase DatA from Agrobacterium tumefaciens C58 possesses a unique halide-stabilising tyrosine residue, Y109, in place of the conventional tryptophan. A variant of DatA with the Y109W mutation was created and the effects of this mutation on the enzyme’s structure and catalytic properties were studied using spectroscopy and pre-steady-state kinetic experiments. (en)
Title	The Effect of a Unique Halide-Stabilising Residue on the Catalytic Properties of Haloalkane Dehalogenase DatA from Agrobacterium tumefaciens C58 The Effect of a Unique Halide-Stabilising Residue on the Catalytic Properties of Haloalkane Dehalogenase DatA from Agrobacterium tumefaciens C58 (en)
skos:prefLabel	The Effect of a Unique Halide-Stabilising Residue on the Catalytic Properties of Haloalkane Dehalogenase DatA from Agrobacterium tumefaciens C58 The Effect of a Unique Halide-Stabilising Residue on the Catalytic Properties of Haloalkane Dehalogenase DatA from Agrobacterium tumefaciens C58 (en)
skos:notation	RIV/00216224:14310/13:00065822!RIV14-MSM-14310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED0001/01/01), P(GAP207/12/0775), P(GAP503/12/0572), P(IAA401630901)
http://linked.open...iv/cisloPeriodika	13
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Prokop, Zbyněk Hasan, Khomaini Fořtová, Andrea Moskalíková, Hana Damborský, Jiří Brezovský, Jan Chaloupková, Radka Gora, Artur Wiktor
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	71336
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/13:00065822
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Haloalkane Dehalogenase (en)
http://linked.open.../riv/klicoveSlovo	Haloalkane Dehalogenase
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[D1FF510E2481]
http://linked.open...i/riv/nazevZdroje	FEBS Journal
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	8 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Strukturně-funkční vztahy haloalkan dehalogenas Construction of a synthetic metabolic pathway for degradation of important environmental pollutant using protein and metabolic engineering CETOCOEN Evolution of substrate specificity in enzymes acting on xenobiotic compounds
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	280
http://linked.open...iv/tvurceVysledku	Brezovský, Jan Chaloupková, Radka Damborský, Jiří Fořtová, Andrea Hasan, Khomaini Nagata, Yuji Prokop, Zbyněk Moskalíková, Hana Gora, Artur Wiktor
http://linked.open...ain/vavai/riv/wos	000320557100016
issn	1742-464X
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/febs.12238
http://localhost/t...ganizacniJednotka	14310

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