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  • Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteria of the genera Photorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves as well for expanding nematodes. Drosophila larvae are suitable insect hosts and part of the tripartite model system we used before to show the importance of haemolymph clotting and eicosanoids during the infection. We used the well-established tripartite model (Drosophila, nematodes, bacteria), DNA chips and bioinformatic tools to compare gene expression in non-infected and infected fly larvae. We focused on the early time point of nematode infection and therefore infected Drosophila larvae using H. bacteriophora harbouring GFP-labelled P. luminescens bacteria.
  • Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteria of the genera Photorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves as well for expanding nematodes. Drosophila larvae are suitable insect hosts and part of the tripartite model system we used before to show the importance of haemolymph clotting and eicosanoids during the infection. We used the well-established tripartite model (Drosophila, nematodes, bacteria), DNA chips and bioinformatic tools to compare gene expression in non-infected and infected fly larvae. We focused on the early time point of nematode infection and therefore infected Drosophila larvae using H. bacteriophora harbouring GFP-labelled P. luminescens bacteria. (en)
Title
  • New insights to insect response to the infection by nematobacterial complex
  • New insights to insect response to the infection by nematobacterial complex (en)
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  • New insights to insect response to the infection by nematobacterial complex
  • New insights to insect response to the infection by nematobacterial complex (en)
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  • RIV/00216224:14310/13:00065683!RIV14-MZE-14310___
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  • 91597
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  • RIV/00216224:14310/13:00065683
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  • Drosophila melanogaster; entomopathogenic nematodes; genome-wide analysis; immunity; infection; symbiotic bacteria (en)
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  • [D84EE203EC5F]
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  • Dobeš, Pavel
  • Hyršl, Pavel
  • Kučerová, Lucie
  • Theopold, Ulrich
  • Wang, Zhi
  • Žurovec, Michal
  • Markus, Robert
  • Arefin, Badrul
http://localhost/t...ganizacniJednotka
  • 14310
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