About: Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm
Description	This study is focused on the intracellular localization of Delta Np73 in medulloblastoma cell lines. Due to a lack of suitable anti-Delta Np73 antibodies, we developed two new rabbit polyclonal antibodies, Delta Np7326 and Delta Np73-27, with sufficient specificity, as demonstrated by immunodetection methods using transiently transfected cell lines. Both of these new antibodies were subsequently used for analysis of the Delta Np73 distribution in medulloblastoma cells using immunofluorescence, immunoblotting and immunogold labeling for transmission electron microscopy. We found a nuclear localization of the Delta Np73 isoform in all of the medulloblastoma cell lines included in this study. Furthermore, a non-random accumulation of the Delta Np73 isoform near the cell nuclei was observable in all of these cell lines. By double-labeling with Delta Np73 and golgin-97, we showed the co-localization of the Delta Np73 isoform with the Golgi apparatus. This study is focused on the intracellular localization of Delta Np73 in medulloblastoma cell lines. Due to a lack of suitable anti-Delta Np73 antibodies, we developed two new rabbit polyclonal antibodies, Delta Np7326 and Delta Np73-27, with sufficient specificity, as demonstrated by immunodetection methods using transiently transfected cell lines. Both of these new antibodies were subsequently used for analysis of the Delta Np73 distribution in medulloblastoma cells using immunofluorescence, immunoblotting and immunogold labeling for transmission electron microscopy. We found a nuclear localization of the Delta Np73 isoform in all of the medulloblastoma cell lines included in this study. Furthermore, a non-random accumulation of the Delta Np73 isoform near the cell nuclei was observable in all of these cell lines. By double-labeling with Delta Np73 and golgin-97, we showed the co-localization of the Delta Np73 isoform with the Golgi apparatus. (en)
Title	Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies (en)
skos:prefLabel	Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies (en)
skos:notation	RIV/00216224:14310/13:00065656!RIV14-MZ0-14310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(NS10218)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Nekulová, Marta Veselská, Renata Štěrba, Jaroslav Zitterbart, Karel Neradil, Jakub Nováková, Lucia
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	80938
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/13:00065656
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	medulloblastoma; deltaNp73; immunocytochemistry; immunoblotting; transmission electron microscopy (en)
http://linked.open.../riv/klicoveSlovo	deltaNp73 immunocytochemistry transmission electron microscopy medulloblastoma immunoblotting
http://linked.open...odStatuVydavatele	ES - Španělské království
http://linked.open...ontrolniKodProRIV	[50588B043DD8]
http://linked.open...i/riv/nazevZdroje	Histology and Histopathology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	The role of p73 protein isoforms in medulloblastoma pathogenesis: an immunocytochemical and microarray study
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	28
http://linked.open...iv/tvurceVysledku	Neradil, Jakub Veselská, Renata Vojtěšek, Bořivoj Zitterbart, Karel Štěrba, Jaroslav Nekulová, Marta Dobrucká, Lucia
http://linked.open...ain/vavai/riv/wos	000320710900011
issn	0213-3911
number of pages	12 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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