About: Engineered recombinant enzyme haloalkane dehalogenase DhaA85

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Thermostable haloalkane dehalogenase DhaA85 prepared by saturated mutagenesis. Mutations targeting as few as four residues lining the access tunnel extended enzyme’s half-life in 40% dimethyl sulfoxide from minutes to weeks (4,000-fold) and increased its melting temperature by 19 C. Protein crystallography and molecular dynamics revealed that the tunnel residue packing is a key determinant of protein stability and the active-site accessibility for co-solvent molecules (red dots). The broad applicability of this concept was verified by analyzing twenty six proteins with buried active sites from all six enzyme classes.The results was made based on the verification of properties. Thermostable haloalkane dehalogenase DhaA85 prepared by saturated mutagenesis. Mutations targeting as few as four residues lining the access tunnel extended enzyme’s half-life in 40% dimethyl sulfoxide from minutes to weeks (4,000-fold) and increased its melting temperature by 19 C. Protein crystallography and molecular dynamics revealed that the tunnel residue packing is a key determinant of protein stability and the active-site accessibility for co-solvent molecules (red dots). The broad applicability of this concept was verified by analyzing twenty six proteins with buried active sites from all six enzyme classes.The results was made based on the verification of properties. (en)
Title	Engineered recombinant enzyme haloalkane dehalogenase DhaA85 Engineered recombinant enzyme haloalkane dehalogenase DhaA85 (en)
skos:prefLabel	Engineered recombinant enzyme haloalkane dehalogenase DhaA85 Engineered recombinant enzyme haloalkane dehalogenase DhaA85 (en)
skos:notation	RIV/00216224:14310/12:00064441!RIV13-MSM-14310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED0001/01/01)
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Prokop, Zbyněk Damborský, Jiří Chaloupková, Radka
http://linked.open.../riv/druhVysledku	G/B - Funkční vzorek
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...onomickeParametry	MU, IČO00216224
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	134404
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/12:00064441
http://linked.open...terniIdentifikace	LL_06_2012
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open...vai/riv/kategorie	A - Do 5 mil. Kč
http://linked.open.../riv/klicovaSlova	haloalkane dehalogenase; DhaA85 (en)
http://linked.open.../riv/klicoveSlovo	DhaA85 haloalkane dehalogenase
http://linked.open...ontrolniKodProRIV	[2C0D1A056C5F]
http://linked.open.../licencniPoplatek	Z - Poskytovatel licence na výsledek nepožaduje v některých případech licenční poplatek
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	CETOCOEN
http://linked.open...UplatneniVysledku	2012
http://linked.open...echnickeParametry	výrobní postup, funkční vzorek byl vyroben na základě ověření vlastností, licenční smlouva nebyla zatím uzavřena.
http://linked.open...iv/tvurceVysledku	Chaloupková, Radka Damborský, Jiří Koudeláková, Táňa Prokop, Zbyněk
http://linked.open...avai/riv/vlastnik	MU
http://linked.open...itiJinymSubjektem	P - Nabytí licence je nutné v některých případech
http://localhost/t...ganizacniJednotka	14310

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