About: Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1111/j.1574-6968.2012.02589.x
Description	The epidemic community-associated methicillin-resistant clone Staphylococcus aureus USA300 is a major source of skin and soft tissue infections and involves strains with a diverse set of resistance genes. In this study, we report efficient transduction of penicillinase and tetracycline resistance plasmids by bacteriophages phi80alpha and phiJB between clinical isolates belonging to the USA300 clone. High transduction frequencies (10-5 to 10-6 CFU/PFU) were observed using phages propagated on donor strains as well as prophages induced from donors by ultraviolet light. Quantitative real-time PCR was employed to detect penicillinase plasmids in transducing phage particles and determine the ratio of transducing particles in phage lysates to infectious phage particles (determined as approximately 1:1700). Successful transfer of plasmids between strains in USA300 clone proves transduction is an effective mechanism for spreading plasmids within the clone. The epidemic community-associated methicillin-resistant clone Staphylococcus aureus USA300 is a major source of skin and soft tissue infections and involves strains with a diverse set of resistance genes. In this study, we report efficient transduction of penicillinase and tetracycline resistance plasmids by bacteriophages phi80alpha and phiJB between clinical isolates belonging to the USA300 clone. High transduction frequencies (10-5 to 10-6 CFU/PFU) were observed using phages propagated on donor strains as well as prophages induced from donors by ultraviolet light. Quantitative real-time PCR was employed to detect penicillinase plasmids in transducing phage particles and determine the ratio of transducing particles in phage lysates to infectious phage particles (determined as approximately 1:1700). Successful transfer of plasmids between strains in USA300 clone proves transduction is an effective mechanism for spreading plasmids within the clone. (en)
Title	Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone (en)
skos:prefLabel	Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone (en)
skos:notation	RIV/00216224:14310/12:00057819!RIV13-GA0-14310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA310/09/0459), Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Doškař, Jiří Růžičková, Vladislava Pantůček, Roman Mašlaňová, Ivana Varga, Marian
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	133578
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/12:00057819
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	antimicrobial drug resistance; penicillinase plasmids; Staphylococcus bacteriophage; lysogeny; horizontal gene transfer; plasmid transduction (en)
http://linked.open.../riv/klicoveSlovo	horizontal gene transfer Staphylococcus bacteriophage antimicrobial drug resistance lysogeny penicillinase plasmids plasmid transduction
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[9A90DAE11A63]
http://linked.open...i/riv/nazevZdroje	FEMS Microbiology Letters
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Role of bacteriophages in horizontal transfer of virulence and drug resistance genes in Staphylococcus aureus
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	332
http://linked.open...iv/tvurceVysledku	Doškař, Jiří Pantůček, Roman Růžičková, Vladislava Mašlaňová, Ivana Kuntová, Lucie Varga, Marian
http://linked.open...ain/vavai/riv/wos	000305334300008
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
issn	0378-1097
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/j.1574-6968.2012.02589.x
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone

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