About: (GTG)5-PCR fingerprinting of lactobacilli isolated from cervix of healthy women

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.springerlink.com/content/4241mw07j07k367w/
Description	A group of lactobacilli isolated from the cervix of 31 healthy women was characterized by (GTG)5-polymerase chain reaction (PCR) fingerprinting in order to evaluate this method for identification of vaginal lactobacilli. Obtained fingerprints were compared with profiles available in an in-house database of the CCM bacteria collection covering type and reference strains of multiple lactic acid bacteria including lactobacilli. Selected strains representing individual clusters were further identified by pheS gene sequencing. In total, six lactobacillus species were found among lactobacilli isolated from the cervix of healthy women. The (GTG)5-PCR method identified Lactobacillus gasseri (11 strains), Lactobacillus fermentum (one), and some of the Lactobacillus jensenii strains (eight out of 11), but failed to identify the remaining strains, including the Lactobacillus crispatus (18), Lactobacillus mucosae (one), and Lactobacillus vaginalis (one) species. L. A group of lactobacilli isolated from the cervix of 31 healthy women was characterized by (GTG)5-polymerase chain reaction (PCR) fingerprinting in order to evaluate this method for identification of vaginal lactobacilli. Obtained fingerprints were compared with profiles available in an in-house database of the CCM bacteria collection covering type and reference strains of multiple lactic acid bacteria including lactobacilli. Selected strains representing individual clusters were further identified by pheS gene sequencing. In total, six lactobacillus species were found among lactobacilli isolated from the cervix of healthy women. The (GTG)5-PCR method identified Lactobacillus gasseri (11 strains), Lactobacillus fermentum (one), and some of the Lactobacillus jensenii strains (eight out of 11), but failed to identify the remaining strains, including the Lactobacillus crispatus (18), Lactobacillus mucosae (one), and Lactobacillus vaginalis (one) species. L. (en)
Title	(GTG)5-PCR fingerprinting of lactobacilli isolated from cervix of healthy women (GTG)5-PCR fingerprinting of lactobacilli isolated from cervix of healthy women (en)
skos:prefLabel	(GTG)5-PCR fingerprinting of lactobacilli isolated from cervix of healthy women (GTG)5-PCR fingerprinting of lactobacilli isolated from cervix of healthy women (en)
skos:notation	RIV/00216224:14310/11:00052382!RIV12-MSM-14310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021622416)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Sedláček, Ivo Švec, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	201601
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/11:00052382
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Lactobacillus; cervix; (GTG)5-PCR; taxonomy; identification (en)
http://linked.open.../riv/klicoveSlovo	(GTG)5-PCR cervix Lactobacillus identification taxonomy
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[22E5C20E188B]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	56
http://linked.open...iv/tvurceVysledku	Sedláček, Ivo Švec, Pavel Chrápavá, Marika Vandamme, Peter
http://linked.open...ain/vavai/riv/wos	000290451500014
http://linked.open...n/vavai/riv/zamer	Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
issn	0015-5632
number of pages	4 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s12223-011-0006-4
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	(GTG)5-PCR fingerprinting of lactobacilli isolated from cervix of healthy women (GTG)5-PCR fingerprinting of lactobacilli isolated from cervix of healthy women

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