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rdf:type
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Description
| - Base composition (expressed most frequently as GC content) is an important classification criterion in microbial research. This might be also the case of plants, however, the data on GC contents in plants are still very poor and some skepticism still exist on the reliability of older estimates as well as on the accuracy of recently cumulating data from flow cytometry (FCM) experiments. FCM enables to measure GC content by comparing results with base specific and base unspecific dyes, most frequently unspecific PI and AT-specific DAPI (Barow et Meister, 2002; Bureš et al., 2004; Šmarda et al., 2008). This measurements are critically dependent on the knowledge of binding length of the used base specific dye (i.e., the number of consecutive pairs of the same type enabling binding of a dye molecule), which is, however, mostly uncertain for many dyes and may vary with specific base patterning in a natural DNA.
- Base composition (expressed most frequently as GC content) is an important classification criterion in microbial research. This might be also the case of plants, however, the data on GC contents in plants are still very poor and some skepticism still exist on the reliability of older estimates as well as on the accuracy of recently cumulating data from flow cytometry (FCM) experiments. FCM enables to measure GC content by comparing results with base specific and base unspecific dyes, most frequently unspecific PI and AT-specific DAPI (Barow et Meister, 2002; Bureš et al., 2004; Šmarda et al., 2008). This measurements are critically dependent on the knowledge of binding length of the used base specific dye (i.e., the number of consecutive pairs of the same type enabling binding of a dye molecule), which is, however, mostly uncertain for many dyes and may vary with specific base patterning in a natural DNA. (en)
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Title
| - Measurements of DNA base composition with flow cytometry and DNA temperature melting
- Measurements of DNA base composition with flow cytometry and DNA temperature melting (en)
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skos:prefLabel
| - Measurements of DNA base composition with flow cytometry and DNA temperature melting
- Measurements of DNA base composition with flow cytometry and DNA temperature melting (en)
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skos:notation
| - RIV/00216224:14310/11:00050575!RIV12-GA0-14310___
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http://linked.open...avai/predkladatel
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA206/09/1405), P(GAP506/11/0890), P(LC06073), Z(MSM0021622416)
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/11:00050575
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - DNA base composition; flow cytometry; DNA temperature melting (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Bureš, Petr
- Šmarda, Petr
- Horová, Lucie
- Šmerda, Jakub
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http://linked.open...n/vavai/riv/zamer
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http://localhost/t...ganizacniJednotka
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