About: Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Labeling of nucleic acids offers broader option for its biochemical and physicochemical studies by the analytical separation methods such as capillary electrophoresis. Suitable labeling allows monitoring of DNA hybridization as well as following of DNA interactions with other biological and/or non-biological compounds. Chemical DNA probe consisting of osmium tetroxide and 2,2'-bipyridine (Os,bipy) is well-known especially for its electrochemical activity. In contrary to vanishing reactivity with purines, Os,bipy reagent creates covalent adducts with pyrimidine bases. The fastest adduct formation was observed namely with thymine. In our work, we analyzed selected pentanucleotides with various number and position of thymine bases and we monitored its reactivity with Os,bipy reagent. Our interest was also taken to ageing of Os,bipy reagent and the role of reagent abundance in mixture with oligonucleotide was studied. Labeling of nucleic acids offers broader option for its biochemical and physicochemical studies by the analytical separation methods such as capillary electrophoresis. Suitable labeling allows monitoring of DNA hybridization as well as following of DNA interactions with other biological and/or non-biological compounds. Chemical DNA probe consisting of osmium tetroxide and 2,2'-bipyridine (Os,bipy) is well-known especially for its electrochemical activity. In contrary to vanishing reactivity with purines, Os,bipy reagent creates covalent adducts with pyrimidine bases. The fastest adduct formation was observed namely with thymine. In our work, we analyzed selected pentanucleotides with various number and position of thymine bases and we monitored its reactivity with Os,bipy reagent. Our interest was also taken to ageing of Os,bipy reagent and the role of reagent abundance in mixture with oligonucleotide was studied. (en)
Title	Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis (en)
skos:prefLabel	Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis (en)
skos:notation	RIV/00216224:14310/10:00043430!RIV11-MSM-14310___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(LC06035), S
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Bittová, Miroslava Trnková, Libuše Fojta, Miroslav Kostečka, Pavel
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	246523
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/10:00043430
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	oligonucleotides osmium tetroxide capillary electrophoresis (en)
http://linked.open.../riv/klicoveSlovo	oligonucleotides osmium tetroxide capillary electrophoresis
http://linked.open...ontrolniKodProRIV	[84ADD02ABB63]
http://linked.open...v/mistoKonaniAkce	Olomouc, Czech Republic
http://linked.open...i/riv/mistoVydani	Olomouc
http://linked.open...i/riv/nazevZdroje	Acta Universitatis Palackianae Olomucensis Chemica 47S
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Bittová, Miroslava Fojta, Miroslav Trnková, Libuše Kostečka, Pavel
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2010-02-08 (xsd:date)
issn	0232-0061
number of pages	158 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Univerzita Palackého v Olomouci
https://schema.org/isbn	978-80-244-2470-5
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis

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