About: IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Overexpression of the histidine kinase CKI1 results into cytokinin-independent activation of two-component signaling (TCS) pathway and cytokinin-like response in hypocotyl explants. CKI1 was found to be involved in the female gametophyte development and procambium activity during vascular tissue formation. Recent data suggest that regulation of CKI1 expression might contribute to the regulation of TCS. We used forward genetics approach to identify the factors regulating the activity of CKI1 promotor. Transgenic lines carrying ProCKI1:uidA construct were mutated by EMS and M2 seedlings were screened for changes in the GUS pattern using automated microscopy (dotSlide) system. The level of CKI1 gene expression in mutants was tested using qRT-PCR and the more detailed analysis of selected mutants is in the progress. Phenotype of these mutants and the influence of spatiotemporal changes in CKI1 expression on the TCS will be analyzed and affected genes will be identified using map-based cloning. Overexpression of the histidine kinase CKI1 results into cytokinin-independent activation of two-component signaling (TCS) pathway and cytokinin-like response in hypocotyl explants. CKI1 was found to be involved in the female gametophyte development and procambium activity during vascular tissue formation. Recent data suggest that regulation of CKI1 expression might contribute to the regulation of TCS. We used forward genetics approach to identify the factors regulating the activity of CKI1 promotor. Transgenic lines carrying ProCKI1:uidA construct were mutated by EMS and M2 seedlings were screened for changes in the GUS pattern using automated microscopy (dotSlide) system. The level of CKI1 gene expression in mutants was tested using qRT-PCR and the more detailed analysis of selected mutants is in the progress. Phenotype of these mutants and the influence of spatiotemporal changes in CKI1 expression on the TCS will be analyzed and affected genes will be identified using map-based cloning. (en)
Title	IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH (en)
skos:prefLabel	IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH (en)
skos:notation	RIV/00216224:14310/09:00043563!RIV11-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LC06034), Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Hejátko, Jan Dobisová, Tereza
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	318335
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/09:00043563
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	CKI1; EMS mutagenesis; forward genetics (en)
http://linked.open.../riv/klicoveSlovo	CKI1 forward genetics EMS mutagenesis
http://linked.open...ontrolniKodProRIV	[8A111FC31C10]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Regulation of morphogenesis of plant cells and organs
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Hejátko, Jan Dobisová, Tereza
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
http://localhost/t...ganizacniJednotka	14310

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