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  • The receiver domain (RD) of a sensor histidine kinase (HKs) catalyses transphosphorylation reaction during the action of HKs in hormonal and abiotic signalling in plants. Crystals of the recombinant RD of the Arabidopsis HK CYTOKININ-INDEPENDENT1 (CKI1RD) have been obtained by the hanging-drop vapour-diffusion method using ammonium sulphate as a precipitant. The crystals diffracted at beamline BW7B of the DORIS-III storage ring to approx. 2.4A. The diffraction has been improved significantly - to at least 2.0A - after applying of a non-water cryoprotectant. The crystals belong to space group C2221 with unit-cell parameters a=54.46, b=99.82, c=79.94 A, the asymmetric unit contains one molecule of the protein. The structure of CKI1RD had been solved by a molecular-replacement.
  • The receiver domain (RD) of a sensor histidine kinase (HKs) catalyses transphosphorylation reaction during the action of HKs in hormonal and abiotic signalling in plants. Crystals of the recombinant RD of the Arabidopsis HK CYTOKININ-INDEPENDENT1 (CKI1RD) have been obtained by the hanging-drop vapour-diffusion method using ammonium sulphate as a precipitant. The crystals diffracted at beamline BW7B of the DORIS-III storage ring to approx. 2.4A. The diffraction has been improved significantly - to at least 2.0A - after applying of a non-water cryoprotectant. The crystals belong to space group C2221 with unit-cell parameters a=54.46, b=99.82, c=79.94 A, the asymmetric unit contains one molecule of the protein. The structure of CKI1RD had been solved by a molecular-replacement. (en)
Title
  • CRYSTALLIZATION AND X-RAY ANALYSIS OF THE RECEIVER DOMAIN OF THE HISTIDINE KINASE CKI1 FROM ARABIDOPSIS THALIANA
  • CRYSTALLIZATION AND X-RAY ANALYSIS OF THE RECEIVER DOMAIN OF THE HISTIDINE KINASE CKI1 FROM ARABIDOPSIS THALIANA (en)
skos:prefLabel
  • CRYSTALLIZATION AND X-RAY ANALYSIS OF THE RECEIVER DOMAIN OF THE HISTIDINE KINASE CKI1 FROM ARABIDOPSIS THALIANA
  • CRYSTALLIZATION AND X-RAY ANALYSIS OF THE RECEIVER DOMAIN OF THE HISTIDINE KINASE CKI1 FROM ARABIDOPSIS THALIANA (en)
skos:notation
  • RIV/00216224:14310/09:00039830!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(LC06034), Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 308617
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/09:00039830
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Crystal sructure; Histidin kinase; cytokinin signaling; CKI1 (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [C33B4868BAC0]
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Hejátko, Jan
  • Janda, Lubomír
  • Marek, Jaromír
  • Pekárová, Blanka
  • Borkovcová, Petra
  • Klumpler, Tomáš
http://linked.open...n/vavai/riv/zamer
http://localhost/t...ganizacniJednotka
  • 14310
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