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  • Introduction: In dairy industry, especially in case of the use of novel probiotic strains, there is a great need for relevant milk culture selection, its proper identification and characterization. In microbiology, modern methods in chemotaxonomy include widely used Matrix Assisted Laser Desorption/Ionization - Time of Flight Mass Spectrometry (MALDI MS). This fast, procedurally simple and sensitive analytical technique provides unique and highly reproducible fingerprints of bacteria (protein/peptide profiles). Profiles of proteins originating from cytoplasmatic membrane and cytoplasm are highly characteristic with great discriminatory power accessible by the analysis of intact bacterial cells. MALDI MS profiles can be effectively used for direct identification of microorganisms and for monitoring of their characteristics (culture changes, metabolic products etc.).
  • Introduction: In dairy industry, especially in case of the use of novel probiotic strains, there is a great need for relevant milk culture selection, its proper identification and characterization. In microbiology, modern methods in chemotaxonomy include widely used Matrix Assisted Laser Desorption/Ionization - Time of Flight Mass Spectrometry (MALDI MS). This fast, procedurally simple and sensitive analytical technique provides unique and highly reproducible fingerprints of bacteria (protein/peptide profiles). Profiles of proteins originating from cytoplasmatic membrane and cytoplasm are highly characteristic with great discriminatory power accessible by the analysis of intact bacterial cells. MALDI MS profiles can be effectively used for direct identification of microorganisms and for monitoring of their characteristics (culture changes, metabolic products etc.). (en)
Title
  • THE USE OF MALDI-TOF MASS SPECTROMETRY WHOLE CELL PROTEIN/PEPTIDE PROFILES FOR IDENTIFICATION AND CHARACTERIZATION OF NOVEL STRAINS WITH PROBIOTIC PROPERTIES
  • THE USE OF MALDI-TOF MASS SPECTROMETRY WHOLE CELL PROTEIN/PEPTIDE PROFILES FOR IDENTIFICATION AND CHARACTERIZATION OF NOVEL STRAINS WITH PROBIOTIC PROPERTIES (en)
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  • THE USE OF MALDI-TOF MASS SPECTROMETRY WHOLE CELL PROTEIN/PEPTIDE PROFILES FOR IDENTIFICATION AND CHARACTERIZATION OF NOVEL STRAINS WITH PROBIOTIC PROPERTIES
  • THE USE OF MALDI-TOF MASS SPECTROMETRY WHOLE CELL PROTEIN/PEPTIDE PROFILES FOR IDENTIFICATION AND CHARACTERIZATION OF NOVEL STRAINS WITH PROBIOTIC PROPERTIES (en)
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  • RIV/00216224:14310/09:00038453!RIV11-MSM-14310___
http://linked.open...avai/riv/aktivita
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  • P(LC06034), S, Z(MSM0021622415), Z(MSM0021622416)
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  • 347899
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  • RIV/00216224:14310/09:00038453
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  • MALDI_MS - chemotaxonomy - lactic acid bacteria (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [7C696FB3F874]
http://linked.open...in/vavai/riv/obor
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  • Dráb, Vladimír
  • Sedláček, Ivo
  • Teshim, Andrea
  • Šedo, Ondrej
  • Švec, Pavel
http://linked.open...n/vavai/riv/zamer
http://localhost/t...ganizacniJednotka
  • 14310
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