About: Developmental changes in phenol-oxidizing activity in the greater wax moth Galleria mellonella L.     Goto   Sponge   NotDistinct   Permalink

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  • Activity of enzymes oxidizing phenolic substrates commonly termed phenoloxidases (POs) was investigated during normal development of Galleria mellonella in haemolymph, intact cuticle and homogenized cuticle. PO activities were determined colorimetrically following chemical activation of prophenoloxidase with acetone or methanol using hydroquinone and pyrogallol as substrates. Hydroquinone with para-positioned hydroxyl group seems to be most suitable for spectrophotometrical detection of phenol-oxidizing activity in various insect tissues because its oxidation products interact only minimally with tissue material. Enzymatic activity changed markedly during development in larvae, pupae and adults. The highest levels of PO in haemolymph were detected using hydroquinone on the fifth day of the last larval instar and at the beginning and at the end of pupal stage.
  • Activity of enzymes oxidizing phenolic substrates commonly termed phenoloxidases (POs) was investigated during normal development of Galleria mellonella in haemolymph, intact cuticle and homogenized cuticle. PO activities were determined colorimetrically following chemical activation of prophenoloxidase with acetone or methanol using hydroquinone and pyrogallol as substrates. Hydroquinone with para-positioned hydroxyl group seems to be most suitable for spectrophotometrical detection of phenol-oxidizing activity in various insect tissues because its oxidation products interact only minimally with tissue material. Enzymatic activity changed markedly during development in larvae, pupae and adults. The highest levels of PO in haemolymph were detected using hydroquinone on the fifth day of the last larval instar and at the beginning and at the end of pupal stage. (en)
Title
  • Developmental changes in phenol-oxidizing activity in the greater wax moth Galleria mellonella L.
  • Developmental changes in phenol-oxidizing activity in the greater wax moth Galleria mellonella L. (en)
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  • Developmental changes in phenol-oxidizing activity in the greater wax moth Galleria mellonella L.
  • Developmental changes in phenol-oxidizing activity in the greater wax moth Galleria mellonella L. (en)
skos:notation
  • RIV/00216224:14310/09:00036599!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita
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  • S
http://linked.open...iv/cisloPeriodika
  • 2
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  • 310100
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  • RIV/00216224:14310/09:00036599
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  • Development; Galleria mellonella; Hydroquinone; Laccase; Phenol-oxidazing activity; Phenoloxidase; Pyrogallol. (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • IT - Italská republika
http://linked.open...ontrolniKodProRIV
  • [8814709B69E5]
http://linked.open...i/riv/nazevZdroje
  • Bulletin of Insectology
http://linked.open...in/vavai/riv/obor
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http://linked.open...v/svazekPeriodika
  • 62
http://linked.open...iv/tvurceVysledku
  • Benešová, Jana
  • Dobeš, Pavel
  • Hyršl, Pavel
http://linked.open...ain/vavai/riv/wos
  • 000272023300017
issn
  • 1721-8861
number of pages
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  • 14310
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