About: Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes.

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About: Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes. Goto Sponge NotDistinct Permalink

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Description	In this work we used capillary zone electrophoresis (CZE) in combination with square wave voltammetry (SWV) at a pyrolytic graphite electrode (PGE) to study reactivity of different pyrimidine residues, thymine (T), uracil (U), cytosine (C) and 5 methyl cytosine (meC), within a pentanucleotide AAAAY (where Y stands for the pyrimidine residue), towards Os,bipy. Since adenine practically does not react with Os,L, Y residues represent the only reactive centre within the pentamers and their reactivity can thus be easily compared. Both techniques revealed the order of the Y reactivities as follows: T>>MeC~U>C. Hence, our results confirmed (a) highly preferential modification of T among all Ys and (b) increased reactivity of MeC, compared to C that was reported by other authors. In this work we used capillary zone electrophoresis (CZE) in combination with square wave voltammetry (SWV) at a pyrolytic graphite electrode (PGE) to study reactivity of different pyrimidine residues, thymine (T), uracil (U), cytosine (C) and 5 methyl cytosine (meC), within a pentanucleotide AAAAY (where Y stands for the pyrimidine residue), towards Os,bipy. Since adenine practically does not react with Os,L, Y residues represent the only reactive centre within the pentamers and their reactivity can thus be easily compared. Both techniques revealed the order of the Y reactivities as follows: T>>MeC~U>C. Hence, our results confirmed (a) highly preferential modification of T among all Ys and (b) increased reactivity of MeC, compared to C that was reported by other authors. (en)
Title	Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes. Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes. (en)
skos:prefLabel	Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes. Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes. (en)
skos:notation	RIV/00216224:14310/09:00036238!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(LC06035)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Bittová, Miroslava Paleček, Emil Trnková, Libuše Fojta, Miroslav
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	312794
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/09:00036238
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA; OsO4 bipy; voltammetry; capillary electrophoresis (en)
http://linked.open.../riv/klicoveSlovo	DNA capillary electrophoresis voltammetry OsO4 bipy
http://linked.open...ontrolniKodProRIV	[71F657B99C79]
http://linked.open...v/mistoKonaniAkce	Jetřichovice
http://linked.open...i/riv/mistoVydani	Ústí nad Labem
http://linked.open...i/riv/nazevZdroje	XXIX. moderní elektrochemické metody - sborník příspěvků
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Bittová, Miroslava Fojta, Miroslav Hianik, Tibor Trnková, Libuše Paleček, Emil Kostečka, Pavel Bartošík, Martin
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2009-01-01 (xsd:date)
number of pages	2 (xsd:int)
http://purl.org/ne...btex#hasPublisher	BEST servis
https://schema.org/isbn	978-80-86238-39-5
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes. Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes. Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes. Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes.

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