About: Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridization     Goto   Sponge   NotDistinct   Permalink

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  • We examined the composition of telomeres in chromosomes of parasitic worms, representatives of the flatworm groups Monogenea and Cestoda (Platyhelminthes), and thorny-headed worms (Syndermata: Acanthocephala) by fluorescence in situ hybridization (FISH) with different telomeric repeat probes. Our results show that the (TTAGGG)n sequence, supposed to be the ancestral telomeric repeat motif of Metazoa, is conserved in Monogenea (Paradiplozoon homoion) and Cestoda (Caryophyllaeus laticeps, Caryophyllaeides fennica, and Nippotaenia mogurndae) but not in Acanthocephala (Pomphorhynchus laevis and Pomphorhynchus tereticollis). In the Pomphorhynchus species, no hybridization signals were obtained with the nematode (TTAGGC)n, arthropod (TTAGG)n, and bdelloid (TGTGGG)n telomeric probes using FISH with their chromosomes and Southern hybridization with P. laevis DNA.
  • We examined the composition of telomeres in chromosomes of parasitic worms, representatives of the flatworm groups Monogenea and Cestoda (Platyhelminthes), and thorny-headed worms (Syndermata: Acanthocephala) by fluorescence in situ hybridization (FISH) with different telomeric repeat probes. Our results show that the (TTAGGG)n sequence, supposed to be the ancestral telomeric repeat motif of Metazoa, is conserved in Monogenea (Paradiplozoon homoion) and Cestoda (Caryophyllaeus laticeps, Caryophyllaeides fennica, and Nippotaenia mogurndae) but not in Acanthocephala (Pomphorhynchus laevis and Pomphorhynchus tereticollis). In the Pomphorhynchus species, no hybridization signals were obtained with the nematode (TTAGGC)n, arthropod (TTAGG)n, and bdelloid (TGTGGG)n telomeric probes using FISH with their chromosomes and Southern hybridization with P. laevis DNA. (en)
Title
  • Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridization
  • Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridization (en)
skos:prefLabel
  • Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridization
  • Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridization (en)
skos:notation
  • RIV/00216224:14310/09:00029571!RIV11-GA0-14310___
http://linked.open...avai/riv/aktivita
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  • P(GA524/07/1610)
http://linked.open...iv/cisloPeriodika
  • 11
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  • 345682
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  • RIV/00216224:14310/09:00029571
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  • Acanthocephala; Platyhelminthes; chromosomes; FISH; telomere (en)
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  • CA - Kanada
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  • [69BF9DD3401B]
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  • Genome
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  • 52
http://linked.open...iv/tvurceVysledku
  • Koubková, Božena
  • Špakulová, Marta
  • Vítková, Magda
  • Bombarová, Marta
http://linked.open...ain/vavai/riv/wos
  • 000254460100027
issn
  • 1480-3321
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  • 14310
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