About: Comparison of five PCR methods for characterization of Streptococcus mutans

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Streptococcus mutans attracts great interest of oral clinical microbiologists because it plays a crucial role in the development of dental caries. Development and evaluation of easy to perform, fast and reliable typing methods is important in all clinical studies. RAPD-PCR with M13 primer as well as rep-PCRs with ERIC, REP, BOX and (GTG)5 primers were performed according to the PCR protocols described previously. All S. mutans strains were typeable by M13 primer and revealed 26 fingerprints ranging from 3 000 to 350 bp. Rep-PCR reactions with ERIC, REP and BOX primers revealed only a few very week or none DNA products; in contrast to these results rep-PCR with the (GTG)5 primer showed 24 fingerprints ranging from 3500 to 200 bp. The (GTG)5-PCR method revealed more complex fingerprints than RAPD-PCR. The clustering revealed by both methods was different and showed comparable discriminative power. The study was supported by projects GACR 310/09/0657 and 1M0528. Streptococcus mutans attracts great interest of oral clinical microbiologists because it plays a crucial role in the development of dental caries. Development and evaluation of easy to perform, fast and reliable typing methods is important in all clinical studies. RAPD-PCR with M13 primer as well as rep-PCRs with ERIC, REP, BOX and (GTG)5 primers were performed according to the PCR protocols described previously. All S. mutans strains were typeable by M13 primer and revealed 26 fingerprints ranging from 3 000 to 350 bp. Rep-PCR reactions with ERIC, REP and BOX primers revealed only a few very week or none DNA products; in contrast to these results rep-PCR with the (GTG)5 primer showed 24 fingerprints ranging from 3500 to 200 bp. The (GTG)5-PCR method revealed more complex fingerprints than RAPD-PCR. The clustering revealed by both methods was different and showed comparable discriminative power. The study was supported by projects GACR 310/09/0657 and 1M0528. (en)
Title	Comparison of five PCR methods for characterization of Streptococcus mutans Comparison of five PCR methods for characterization of Streptococcus mutans (en)
skos:prefLabel	Comparison of five PCR methods for characterization of Streptococcus mutans Comparison of five PCR methods for characterization of Streptococcus mutans (en)
skos:notation	RIV/00216224:14310/09:00029411!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(1M0528), P(GA310/09/0657)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Nováková, Dana Sedláček, Ivo Švec, Pavel Kukletová, Martina
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	307712
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/09:00029411
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Streptococcus mutans; RAPD-PCR; rep-PCR; taxonomy (en)
http://linked.open.../riv/klicoveSlovo	taxonomy rep-PCR Streptococcus mutans RAPD-PCR
http://linked.open...ontrolniKodProRIV	[60D3AF1A97CD]
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Dental research centre Molecular taxonomy of Gram-positive bacteria isolated from early childhood caries
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Sedláček, Ivo Švec, Pavel Kukletová, Martina Nováková, Dana
http://localhost/t...ganizacniJednotka	14310

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