About: New CAPS Marker for Selection of a Barley Powdery Mildew Resistance Gene in the Mla Locus     Goto   Sponge   NotDistinct   Permalink

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Description
  • In PI466495, a powdery mildew resistance source of wild barley (Hordeum vulgare ssp. spontaneum), one gene conferring powdery mildew resistance was identified in the Mla locus. In this paper, the RGH1a gene sequence was used as source for the development of a cleaved amplified polymorphic sequence (CAPS) marker. Cosegregation between this marker and powdery mildew resistance was analysed by specific DNA fragments associated with each allele of the gene using 286 F2 plants derived from a cross between winter barley (H. vulgare L.) variety Tiffany and PI466495. For the codominant marker RGH1aI1a, three fragments, 370 bp, 82 bp and 59 bp in size, were amplified from F2 plants exhibiting resistance reaction types 0 and 0/1 to powdery mildew; whereas two fragments, 429 bp and 82 bp in size, were amplified in susceptible plants. Simple procedures based on polymerase chain reaction and restriction enzyme digestion allowed for identifying the plants susceptible to powdery mildew (Blumeria graminis f. sp.
  • In PI466495, a powdery mildew resistance source of wild barley (Hordeum vulgare ssp. spontaneum), one gene conferring powdery mildew resistance was identified in the Mla locus. In this paper, the RGH1a gene sequence was used as source for the development of a cleaved amplified polymorphic sequence (CAPS) marker. Cosegregation between this marker and powdery mildew resistance was analysed by specific DNA fragments associated with each allele of the gene using 286 F2 plants derived from a cross between winter barley (H. vulgare L.) variety Tiffany and PI466495. For the codominant marker RGH1aI1a, three fragments, 370 bp, 82 bp and 59 bp in size, were amplified from F2 plants exhibiting resistance reaction types 0 and 0/1 to powdery mildew; whereas two fragments, 429 bp and 82 bp in size, were amplified in susceptible plants. Simple procedures based on polymerase chain reaction and restriction enzyme digestion allowed for identifying the plants susceptible to powdery mildew (Blumeria graminis f. sp. (en)
Title
  • New CAPS Marker for Selection of a Barley Powdery Mildew Resistance Gene in the Mla Locus
  • New CAPS Marker for Selection of a Barley Powdery Mildew Resistance Gene in the Mla Locus (en)
skos:prefLabel
  • New CAPS Marker for Selection of a Barley Powdery Mildew Resistance Gene in the Mla Locus
  • New CAPS Marker for Selection of a Barley Powdery Mildew Resistance Gene in the Mla Locus (en)
skos:notation
  • RIV/00216224:14310/09:00029161!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA522/06/0608), Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 329324
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/09:00029161
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Blumeria graminis f. sp. hordei; DNA markers; Hordeum vulgare ssp. spontaneum; resistance gene homolog (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • HU - Maďarsko
http://linked.open...ontrolniKodProRIV
  • [B01EBFFFF574]
http://linked.open...i/riv/nazevZdroje
  • Cereal Research Communications
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 37
http://linked.open...iv/tvurceVysledku
  • Dreiseitl, Antonín
  • Řepková, Jana
  • Lízal, Pavel
http://linked.open...ain/vavai/riv/wos
  • 000264755800011
http://linked.open...n/vavai/riv/zamer
issn
  • 0133-3720
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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