About: Nanopolyperoxidase – a universal system for simultaneous colorimetric signal enhancement and nanoscale visualization of antibody labeling

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Nanopolyperoxidase – a universal system for simultaneous colorimetric signal enhancement and nanoscale visualization of antibody labeling Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	During past decades, a variety of signal amplification approaches in heterogeneous affinity immunoassays (including ELISA) has been developed. Those procedures are usually based either on recirculation enzymatic or repeated multiplication of an enzyme label via avidin-biotin system. From practical point of view, the multiplication procedure should not change experimental part of method, e.g. number of steps in procedure or solution content should not be changed. For this reason, an idea of nanopolyperoxidase (NPP) has originated. The principle is simple: gold nanoparticles are modified with an antibody as well as with labeling enzyme (Horseradish peroxidase; ration 2:8). Modified nanoparticle is stabilized in a PEG solution. Prepared NPP was studied using various methods. Atomic Force Microscopy (AFM) was used for characterization of structure and morphology in a nanometer scale (see image below). During past decades, a variety of signal amplification approaches in heterogeneous affinity immunoassays (including ELISA) has been developed. Those procedures are usually based either on recirculation enzymatic or repeated multiplication of an enzyme label via avidin-biotin system. From practical point of view, the multiplication procedure should not change experimental part of method, e.g. number of steps in procedure or solution content should not be changed. For this reason, an idea of nanopolyperoxidase (NPP) has originated. The principle is simple: gold nanoparticles are modified with an antibody as well as with labeling enzyme (Horseradish peroxidase; ration 2:8). Modified nanoparticle is stabilized in a PEG solution. Prepared NPP was studied using various methods. Atomic Force Microscopy (AFM) was used for characterization of structure and morphology in a nanometer scale (see image below). (en)
Title	Nanopolyperoxidase – a universal system for simultaneous colorimetric signal enhancement and nanoscale visualization of antibody labeling Nanopolyperoxidase – a universal system for simultaneous colorimetric signal enhancement and nanoscale visualization of antibody labeling (en)
skos:prefLabel	Nanopolyperoxidase – a universal system for simultaneous colorimetric signal enhancement and nanoscale visualization of antibody labeling Nanopolyperoxidase – a universal system for simultaneous colorimetric signal enhancement and nanoscale visualization of antibody labeling (en)
skos:notation	RIV/00216224:14310/08:00052277!RIV12-MSM-14310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(2B06056)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Skládal, Petr Frnková, Kateřina
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	381609
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/08:00052277
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	antibody enzyme-label blotting ELISA (en)
http://linked.open.../riv/klicoveSlovo	antibody enzyme-label blotting ELISA
http://linked.open...ontrolniKodProRIV	[DD750127AA13]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	5th international interdisciplinary meeting on bioanalysis CECE 2008
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Nanotechnological and bioanalytical detection of the DNA damage
http://linked.open...UplatneniVysledku	2008
http://linked.open...iv/tvurceVysledku	Skládal, Petr Suchánek, Miloslav Přibyl, Jan Frnková, Kateřina
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2008-11-24 (xsd:date)
number of pages	2 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Institute of Analytical Chemisty AS CR
https://schema.org/isbn	978-80-254-3194-8
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Nanopolyperoxidase – a universal system for simultaneous colorimetric signal enhancement and nanoscale visualization of antibody labeling Nanopolyperoxidase – a universal system for simultaneous colorimetric signal enhancement and nanoscale visualization of antibody labeling

Faceted Search & Find service v1.16.116 as of Feb 22 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3239 as of Feb 22 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 82 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software