About: Preparation of protein conjugates with highly hydrophobic molecules and their use in heterogeneous immunoassay

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Chromatogpraphic methods coupled to a mass spectroscopy are widely used in analysi of PAHs and their metabolites, as those methods provide high sensitivity and selectivity, combined with excellent robustness. Immunoanalytical methods, such as ELISA, offer interesting possibility to detect PAHs at low levels with low expanses. Moreover, development of an antibody with specifity to polyaromatic hydrocarbons offers a possibility to detect DNA-PAH adducts. Preparation and characterization of immunoconjugates of polyaromatic hydrocarbon model compound (pyrenebutyric acid) with proteins (carrier proteins and enzymes) is described (shown as scheme on this page). PAHs compounds as highly lipophilic molecules can not be conjugated to a protein structure in a single step procedure, however, step-by-step changing of conjugation and purification steps needs to be employed in this way. Use of prepared conjugates in construction of direct and indirect ELISA method is shown, too. Chromatogpraphic methods coupled to a mass spectroscopy are widely used in analysi of PAHs and their metabolites, as those methods provide high sensitivity and selectivity, combined with excellent robustness. Immunoanalytical methods, such as ELISA, offer interesting possibility to detect PAHs at low levels with low expanses. Moreover, development of an antibody with specifity to polyaromatic hydrocarbons offers a possibility to detect DNA-PAH adducts. Preparation and characterization of immunoconjugates of polyaromatic hydrocarbon model compound (pyrenebutyric acid) with proteins (carrier proteins and enzymes) is described (shown as scheme on this page). PAHs compounds as highly lipophilic molecules can not be conjugated to a protein structure in a single step procedure, however, step-by-step changing of conjugation and purification steps needs to be employed in this way. Use of prepared conjugates in construction of direct and indirect ELISA method is shown, too. (en)
Title	Preparation of protein conjugates with highly hydrophobic molecules and their use in heterogeneous immunoassay Preparation of protein conjugates with highly hydrophobic molecules and their use in heterogeneous immunoassay (en)
skos:prefLabel	Preparation of protein conjugates with highly hydrophobic molecules and their use in heterogeneous immunoassay Preparation of protein conjugates with highly hydrophobic molecules and their use in heterogeneous immunoassay (en)
skos:notation	RIV/00216224:14310/08:00052275!RIV12-MSM-14310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(2B06056)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Skládal, Petr Přibyl, Jan Frnková, Kateřina
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	389039
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/08:00052275
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	bioconjugates immunization ELISA (en)
http://linked.open.../riv/klicoveSlovo	bioconjugates immunization ELISA
http://linked.open...ontrolniKodProRIV	[6C96C9740BEA]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	5th International Interdisciplinary Meeting on Bioanalysis CECE2008
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Nanotechnological and bioanalytical detection of the DNA damage
http://linked.open...UplatneniVysledku	2008
http://linked.open...iv/tvurceVysledku	Skládal, Petr Přibyl, Jan Frnková, Kateřina Suchácek, Miloslav
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2008-11-25 (xsd:date)
number of pages	2 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Institute of Analytical Chemisty AS CR
https://schema.org/isbn	978-80-254-3194-8
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Preparation of protein conjugates with highly hydrophobic molecules and their use in heterogeneous immunoassay Preparation of protein conjugates with highly hydrophobic molecules and their use in heterogeneous immunoassay

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