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  • The general application of ethidium monoazide (EMA) is based on penetration into dead cells with copromised membrane integrity. DNA bound to EMA cannot be PCR amplified. Experiments were performed using probiotic bacterial strein Lactobacillus paracasei subsp. paracasei CCDM 211/06. The lactobacillus species specific primers were used for DNA amplification. The PCR products (250 bp) were separated using electrophoresis in 1.8 % agarose gel. The method was applied for live and dead lactobacillus cells identification in dairy products.
  • The general application of ethidium monoazide (EMA) is based on penetration into dead cells with copromised membrane integrity. DNA bound to EMA cannot be PCR amplified. Experiments were performed using probiotic bacterial strein Lactobacillus paracasei subsp. paracasei CCDM 211/06. The lactobacillus species specific primers were used for DNA amplification. The PCR products (250 bp) were separated using electrophoresis in 1.8 % agarose gel. The method was applied for live and dead lactobacillus cells identification in dairy products. (en)
Title
  • Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells
  • Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells (en)
skos:prefLabel
  • Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells
  • Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells (en)
skos:notation
  • RIV/00216224:14310/08:00026233!RIV10-MZE-14310___
http://linked.open...avai/riv/aktivita
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  • P(1G57037), Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 385239
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/08:00026233
http://linked.open...riv/jazykVysledku
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  • ethidium monoazide; dead and live bacterial cells (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [CA1520B334DC]
http://linked.open...v/mistoKonaniAkce
  • Vysoké Tatry, Slovensko
http://linked.open...i/riv/mistoVydani
  • Bratislava
http://linked.open...i/riv/nazevZdroje
  • International Probiotic Conference. Pediatria 2, 2008.
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  • Rittich, Bohuslav
  • Trachtová, Štěpánka
  • Španová, Alena
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
issn
  • 1336-863X
number of pages
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  • Univerzita P.J. Šafárika, Košice
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  • 14310
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