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Description
| - The aim of this study was to clarify taxonomic position of a group of 29 biochemically presumptively identified Staphylococcus strains deposited in the Czech Collection of Microorganisms (http://www.sci.muni.cz/ccm/). Biotyping was done by API Staph, API Zym commercial kits and conventional tests. More detailed characterization was performed by ribotyping with both EcoRI and HindIII restriction endonucleases, whole-cell protein analysis and the (GTG)5-PCR fingerprinting. All applied methods congruently separated most of analysed strains and assigned them as S. haemolyticus, S. equorum, S. saprophyticus subsp. saprophyticus, S. kloosii, S. cohnii subsp. cohnii and S. epidermidis species. However these methods did not provide congruent results in several strains. Ribotyping as well as protein analysis failed to unambiguously distinguish some oxidase positive S. sciuri, S. fleurettii and S. lentus strains revealing highly similar patterns; but they were clearly separated by the (GTG)5-PCR fingerprinting.
- The aim of this study was to clarify taxonomic position of a group of 29 biochemically presumptively identified Staphylococcus strains deposited in the Czech Collection of Microorganisms (http://www.sci.muni.cz/ccm/). Biotyping was done by API Staph, API Zym commercial kits and conventional tests. More detailed characterization was performed by ribotyping with both EcoRI and HindIII restriction endonucleases, whole-cell protein analysis and the (GTG)5-PCR fingerprinting. All applied methods congruently separated most of analysed strains and assigned them as S. haemolyticus, S. equorum, S. saprophyticus subsp. saprophyticus, S. kloosii, S. cohnii subsp. cohnii and S. epidermidis species. However these methods did not provide congruent results in several strains. Ribotyping as well as protein analysis failed to unambiguously distinguish some oxidase positive S. sciuri, S. fleurettii and S. lentus strains revealing highly similar patterns; but they were clearly separated by the (GTG)5-PCR fingerprinting. (en)
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Title
| - Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms
- Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms (en)
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skos:prefLabel
| - Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms
- Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms (en)
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skos:notation
| - RIV/00216224:14310/07:00023007!RIV10-MSM-14310___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/07:00023007
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Staphylococcus sp.; ribotyping; whole-cell protein analysis; rep-PCR (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Sedláček, Ivo
- Švec, Pavel
- Nováková, Dana
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http://linked.open...n/vavai/riv/zamer
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http://localhost/t...ganizacniJednotka
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