About: Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry

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About: Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	,2,3-trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes catalysing the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been considered inactive with TCP since the reaction can not be easily detected by conventional analytical methods. Here we demonstrate detection of the weak activity (kcat = 0.005 s-1) of LinB with TCP using X-ray crystallography and microcalorimetry. This observation makes LinB a useful starting material for development of new biocatalyst towards TCP by protein engineering. Microcalorimetry is proposed to be a universal method for detection of weak enzymatic activities. Detection of these activities is becoming increasingly important for engineering of novel biocatalysts using the scaffolds of proteins with promiscuous activities. ,2,3-trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes catalysing the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been considered inactive with TCP since the reaction can not be easily detected by conventional analytical methods. Here we demonstrate detection of the weak activity (kcat = 0.005 s-1) of LinB with TCP using X-ray crystallography and microcalorimetry. This observation makes LinB a useful starting material for development of new biocatalyst towards TCP by protein engineering. Microcalorimetry is proposed to be a universal method for detection of weak enzymatic activities. Detection of these activities is becoming increasingly important for engineering of novel biocatalysts using the scaffolds of proteins with promiscuous activities. (en)
Title	Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry (en)
skos:prefLabel	Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry (en)
skos:notation	RIV/00216224:14310/07:00022815!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021622412)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Prokop, Zbyněk Damborský, Jiří Monincová, Marta Nagata, Yuji
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	461135
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/07:00022815
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	1; 2; 3-trichloropropane (TCP); Haloalkane dehalogenases; LinB from Sphingobium japonicum UT26; X-ray crystallography; microcalorimetry (en)
http://linked.open.../riv/klicoveSlovo	X-ray crystallography 1 2 3-trichloropropane (TCP) LinB from Sphingobium japonicum UT26 Haloalkane dehalogenases microcalorimetry
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[D054185BFDC3]
http://linked.open...i/riv/nazevZdroje	Applied and Environmental Microbiology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	73
http://linked.open...iv/tvurceVysledku	Damborský, Jiří Nagata, Yuji Prokop, Zbyněk Monincová, Marta Vevodová, Jitka
http://linked.open...ain/vavai/riv/wos	000245156800039
http://linked.open...n/vavai/riv/zamer	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
issn	0099-2240
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry

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