About: Misidentification of Streptococcus mutans strains isolated from dental caries as Lactobacillus sp.     Goto   Sponge   NotDistinct   Permalink

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  • A group of eight Gram-positive rods was isolated during a screening of Lactobacillus spp. in dental caries. All strains were isolated on MRS agar and revealed cell and colony morphology typical for the genus Lactobacillus. Phenotypical identification using conventional tests and API 50CH kit assigned all strains as Lactobacillus sp. biochemically close to Lactobacillus brevis. Further investigation using rep-PCR with the (GTG)5 primer separated analysed group into a homogeneous cluster clearly separated from all lactobacilli included in the in-house CCM database but their (GTG)5-PCR fingerprints matched well Streptococcus mutans representatives. Further comparison of biochemical test results with characteristics stated for S. mutans clearly confirmed all strains as members of this species. Our results showed that S. mutans strains retrieved from dental caries can be misidentified as Lactobacillus sp.
  • A group of eight Gram-positive rods was isolated during a screening of Lactobacillus spp. in dental caries. All strains were isolated on MRS agar and revealed cell and colony morphology typical for the genus Lactobacillus. Phenotypical identification using conventional tests and API 50CH kit assigned all strains as Lactobacillus sp. biochemically close to Lactobacillus brevis. Further investigation using rep-PCR with the (GTG)5 primer separated analysed group into a homogeneous cluster clearly separated from all lactobacilli included in the in-house CCM database but their (GTG)5-PCR fingerprints matched well Streptococcus mutans representatives. Further comparison of biochemical test results with characteristics stated for S. mutans clearly confirmed all strains as members of this species. Our results showed that S. mutans strains retrieved from dental caries can be misidentified as Lactobacillus sp. (en)
Title
  • Misidentification of Streptococcus mutans strains isolated from dental caries as Lactobacillus sp.
  • Misidentification of Streptococcus mutans strains isolated from dental caries as Lactobacillus sp. (en)
skos:prefLabel
  • Misidentification of Streptococcus mutans strains isolated from dental caries as Lactobacillus sp.
  • Misidentification of Streptococcus mutans strains isolated from dental caries as Lactobacillus sp. (en)
skos:notation
  • RIV/00216224:14310/07:00022762!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1M0528), Z(MSM0021622416)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 434029
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/07:00022762
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Streptococcus mutans; Lactobacillus sp.; identification; dental caries (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [BA0D5D3689BE]
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Sedláček, Ivo
  • Švec, Pavel
  • Kukletová, Martina
  • Nováková, Dana
  • Žáčková, Lenka
http://linked.open...n/vavai/riv/zamer
http://localhost/t...ganizacniJednotka
  • 14310
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