About: Molecular analysis of Frigida (Fri) gene in late-flowering genotypes of Arabidopsis thaliana

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Flowering time is stimulated by both environmental (light, temperature) and endogenous signals. FLC and FRI genes are the most important endogenous signals controlling onset of flowering in natural populations. FLC is a significant flowering repressor under the control of the FRI gene. Interaction of both genes is the reason of lateness in natural populations. In the FRI gene, two frequent deletions responsible for early-flowering phenotype, Ler- and Col-type are known. These deletions have been identified only in early-flowering plants but never in late-flowering plants. Therefore, our work was focused on molecular analysis of FRI gene in late-flowering ecotypes derived from natural populations in the Czech Republic (Je-4, Je-18, Je-27, Je-28 and Hod) and in four late-flowering mutants (dn, L4, Spi and M73). For this purpose five PCR primers that cover the complete sequence of the FRI gene including the promoter were used. Deletions or insertions were detected by agarose or polyacrylamide gels. Flowering time is stimulated by both environmental (light, temperature) and endogenous signals. FLC and FRI genes are the most important endogenous signals controlling onset of flowering in natural populations. FLC is a significant flowering repressor under the control of the FRI gene. Interaction of both genes is the reason of lateness in natural populations. In the FRI gene, two frequent deletions responsible for early-flowering phenotype, Ler- and Col-type are known. These deletions have been identified only in early-flowering plants but never in late-flowering plants. Therefore, our work was focused on molecular analysis of FRI gene in late-flowering ecotypes derived from natural populations in the Czech Republic (Je-4, Je-18, Je-27, Je-28 and Hod) and in four late-flowering mutants (dn, L4, Spi and M73). For this purpose five PCR primers that cover the complete sequence of the FRI gene including the promoter were used. Deletions or insertions were detected by agarose or polyacrylamide gels. (en)
Title	Molecular analysis of Frigida (Fri) gene in late-flowering genotypes of Arabidopsis thaliana Molecular analysis of Frigida (Fri) gene in late-flowering genotypes of Arabidopsis thaliana (en)
skos:prefLabel	Molecular analysis of Frigida (Fri) gene in late-flowering genotypes of Arabidopsis thaliana Molecular analysis of Frigida (Fri) gene in late-flowering genotypes of Arabidopsis thaliana (en)
skos:notation	RIV/00216224:14310/07:00022293!RIV11-MSM-14310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Lízal, Pavel Řepková, Jana Cetkovská, Kateřina Relichová, Jiřina
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	434830
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/07:00022293
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Arabidopsis thaliana; flowering time; mutation; RT-PCR (en)
http://linked.open.../riv/klicoveSlovo	Arabidopsis thaliana RT-PCR flowering time mutation
http://linked.open...ontrolniKodProRIV	[F0218E1F5810]
http://linked.open...v/mistoKonaniAkce	Stará Lesná
http://linked.open...i/riv/mistoVydani	Stará Lesná
http://linked.open...i/riv/nazevZdroje	Plant biotechnology: impact on high quality plant reproduction
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2007
http://linked.open...iv/tvurceVysledku	Řepková, Jana Cetkovská, Kateřina Lízal, Pavel Relichová, Jiřina
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2007-01-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
number of pages	2 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Ústav genetiky a biotechnológií rastlín SAV
https://schema.org/isbn	978-80-89088-51-5
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Molecular analysis of Frigida (Fri) gene in late-flowering genotypes of Arabidopsis thaliana Molecular analysis of Frigida (Fri) gene in late-flowering genotypes of Arabidopsis thaliana

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