About: Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Magnetite nanoparticles ca. 14 nm in diameter were obtained by chemical coprecipitation of Fe(II) and Fe(III) salts with ammonia in the presence of poly(ethylene glycol) (PEG). Magnetic poly(glycidyl methacrylate) (PGMA) microspheres were prepared by dispersion polymerization in poly(vinylpyrrolidone) (PVP)-stabilized aqueous ethanol with PEG-coated magnetite nanoparticles. Magnetic PGMA microspheres were hydrolyzed and carboxyl groups introduced by oxidation with KMnO4. Such a carrier was used for isolation of DNA. The particles reversibly bound DNA in the presence of high concentration of PEG 6,000 and sodium chloride. Bacterial DNA was isolated from crude cell lysates of different dairy products (butter milk, cheese, yoghurt), probiotic tablets or from cell lyophilisates. The presence of Bifidobacterium and Lactobacillus DNA in samples was identified by PCR amplification. Magnetite nanoparticles ca. 14 nm in diameter were obtained by chemical coprecipitation of Fe(II) and Fe(III) salts with ammonia in the presence of poly(ethylene glycol) (PEG). Magnetic poly(glycidyl methacrylate) (PGMA) microspheres were prepared by dispersion polymerization in poly(vinylpyrrolidone) (PVP)-stabilized aqueous ethanol with PEG-coated magnetite nanoparticles. Magnetic PGMA microspheres were hydrolyzed and carboxyl groups introduced by oxidation with KMnO4. Such a carrier was used for isolation of DNA. The particles reversibly bound DNA in the presence of high concentration of PEG 6,000 and sodium chloride. Bacterial DNA was isolated from crude cell lysates of different dairy products (butter milk, cheese, yoghurt), probiotic tablets or from cell lyophilisates. The presence of Bifidobacterium and Lactobacillus DNA in samples was identified by PCR amplification. (en)
Title	Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products (en)
skos:prefLabel	Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products (en)
skos:notation	RIV/00216224:14310/07:00020478!RIV10-GA0-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA525/05/0311), Z(AV0Z40500505)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Španová, Alena Rittich, Bohuslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	412642
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/07:00020478
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	magnetic particles; DNA; magnetite; PCR; dispersion polymerization; bacterial DNA assay (en)
http://linked.open.../riv/klicoveSlovo	DNA PCR magnetite bacterial DNA assay magnetic particles dispersion polymerization
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[6D8192BC0512]
http://linked.open...i/riv/nazevZdroje	Journal of Magnetism and Magnetic Materials
http://linked.open...in/vavai/riv/obor	CD
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Rapid methods for detection of pathogenic Yersinia enterocolitica in foods
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	311
http://linked.open...iv/tvurceVysledku	Horák, Daniel Rittich, Bohuslav Španová, Alena
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie
issn	0304-8853
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products

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