About: Characterization of staphylococcal type strains by whole-cell protein analysis and (GTG)5-PCR fingerprinting.

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About: Characterization of staphylococcal type strains by whole-cell protein analysis and (GTG)5-PCR fingerprinting. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A group of 47 type strains representing all validly described staphylococcal species and subspecies was characterized by whole-cell protein analysis by using sodium dodecylsulfate-polyacrylamide gel electrophoresis and rep-PCR fingerprinting with the (GTG)5 primer. All strains were well typeable and revealed unique fingerprints by both methods. The individual staphylococcal type strains were differentiated on the level of 74 % using the protein analysis, with exception of novobiocin-resistant species, and on the level of 68 % by using the (GTG)5-PCR fingerprinting. Generally, all subspecies of the individual species showed close protein profiles that were grouped into individual clusters. Similarly, but with a few exceptions, the individual subspecies were separated into the distinct clusters using the (GTG)5-PCR fingerprinting. No clear correlation between the clustering obtained by both methods and the phylogenetic relationships of the analysed species was observed. A group of 47 type strains representing all validly described staphylococcal species and subspecies was characterized by whole-cell protein analysis by using sodium dodecylsulfate-polyacrylamide gel electrophoresis and rep-PCR fingerprinting with the (GTG)5 primer. All strains were well typeable and revealed unique fingerprints by both methods. The individual staphylococcal type strains were differentiated on the level of 74 % using the protein analysis, with exception of novobiocin-resistant species, and on the level of 68 % by using the (GTG)5-PCR fingerprinting. Generally, all subspecies of the individual species showed close protein profiles that were grouped into individual clusters. Similarly, but with a few exceptions, the individual subspecies were separated into the distinct clusters using the (GTG)5-PCR fingerprinting. No clear correlation between the clustering obtained by both methods and the phylogenetic relationships of the analysed species was observed. (en)
Title	Characterization of staphylococcal type strains by whole-cell protein analysis and (GTG)5-PCR fingerprinting. Characterization of staphylococcal type strains by whole-cell protein analysis and (GTG)5-PCR fingerprinting. (en)
skos:prefLabel	Characterization of staphylococcal type strains by whole-cell protein analysis and (GTG)5-PCR fingerprinting. Characterization of staphylococcal type strains by whole-cell protein analysis and (GTG)5-PCR fingerprinting. (en)
skos:notation	RIV/00216224:14310/06:00021871!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021622416)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Nováková, Dana Sedláček, Ivo Švec, Pavel
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	468422
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/06:00021871
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	staphylococcal type strains; whole-cell protein analysis; (GTG)5-PCR fingerprinting (en)
http://linked.open.../riv/klicoveSlovo	whole-cell protein analysis (GTG)5-PCR fingerprinting staphylococcal type strains
http://linked.open...ontrolniKodProRIV	[313D70215E64]
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2006
http://linked.open...iv/tvurceVysledku	Sedláček, Ivo Švec, Pavel Nováková, Dana
http://linked.open...n/vavai/riv/zamer	Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
http://localhost/t...ganizacniJednotka	14310

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