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About: A CONTINUOUS MODE OF OPERATION FOR LARGE VOLUME DOSING IN ANALYTICAL CARRIER AMPHOLYTE–FREE ISOELECTRIC FOCUSING OF PROTEINS APPLIED TO OFF–LINE DETECTION OF FRACTIONS Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Mass spectrometry (MS) is being increasingly used for analysis of proteome complex samples. To remove matrix interferences and to concentrate analytes prior to a MS measurement, sample preparation is often necessary. A useful method for this purpose is Carrier Ampholyte Free-Isoelectric Focusing (CAF-IEF). In this paper CAF-IEF of ampholytes was performed on a commercial apparatus EA101 (Villa Labeco, Slovakia) equipped with a specially made column for samples of large volumes (up to 0.5 ml). A new continuous mode without voltage interruption or electrolyte replacement was developed. In this mode, a low molecular pI marker (PIM 7.4), low concentrations of myoglobin and insulin (16 mg/l), respectively, were concentrated, and then 5-ul fractions collected for off-line analyses. The total time of focusing was 66 minutes. Concentration of PIM 7.4 in the fractions was increased up to 75 times (determined by UV-VIS spectrometry). Mass spectrometry (MS) is being increasingly used for analysis of proteome complex samples. To remove matrix interferences and to concentrate analytes prior to a MS measurement, sample preparation is often necessary. A useful method for this purpose is Carrier Ampholyte Free-Isoelectric Focusing (CAF-IEF). In this paper CAF-IEF of ampholytes was performed on a commercial apparatus EA101 (Villa Labeco, Slovakia) equipped with a specially made column for samples of large volumes (up to 0.5 ml). A new continuous mode without voltage interruption or electrolyte replacement was developed. In this mode, a low molecular pI marker (PIM 7.4), low concentrations of myoglobin and insulin (16 mg/l), respectively, were concentrated, and then 5-ul fractions collected for off-line analyses. The total time of focusing was 66 minutes. Concentration of PIM 7.4 in the fractions was increased up to 75 times (determined by UV-VIS spectrometry). (en)
Title	A CONTINUOUS MODE OF OPERATION FOR LARGE VOLUME DOSING IN ANALYTICAL CARRIER AMPHOLYTE–FREE ISOELECTRIC FOCUSING OF PROTEINS APPLIED TO OFF–LINE DETECTION OF FRACTIONS A CONTINUOUS MODE OF OPERATION FOR LARGE VOLUME DOSING IN ANALYTICAL CARRIER AMPHOLYTE–FREE ISOELECTRIC FOCUSING OF PROTEINS APPLIED TO OFF–LINE DETECTION OF FRACTIONS (en)
skos:prefLabel	A CONTINUOUS MODE OF OPERATION FOR LARGE VOLUME DOSING IN ANALYTICAL CARRIER AMPHOLYTE–FREE ISOELECTRIC FOCUSING OF PROTEINS APPLIED TO OFF–LINE DETECTION OF FRACTIONS A CONTINUOUS MODE OF OPERATION FOR LARGE VOLUME DOSING IN ANALYTICAL CARRIER AMPHOLYTE–FREE ISOELECTRIC FOCUSING OF PROTEINS APPLIED TO OFF–LINE DETECTION OF FRACTIONS (en)
skos:notation	RIV/00216224:14310/06:00016304!RIV10-GA0-14310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA305/03/1100)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Pazourek, Jiří Budilová, Jana Pospíchal, Jan Krásenský, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	463472
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/06:00016304
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	capillary isoelectric focusing; neutralization reaction boundary; proteins; fractionation; injected large volume sample (en)
http://linked.open.../riv/klicoveSlovo	fractionation capillary isoelectric focusing proteins injected large volume sample neutralization reaction boundary
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[646BA4B89FB7]
http://linked.open...i/riv/nazevZdroje	Journal of Separation Science
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Synthesis and study of properties of neuroprotective peptide analogues derived of humanin
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	29
http://linked.open...iv/tvurceVysledku	Pazourek, Jiří Budilová, Jana Pospíchal, Jan Krásenský, Pavel
issn	1615-9306
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	A CONTINUOUS MODE OF OPERATION FOR LARGE VOLUME DOSING IN ANALYTICAL CARRIER AMPHOLYTE–FREE ISOELECTRIC FOCUSING OF PROTEINS APPLIED TO OFF–LINE DETECTION OF FRACTIONS A CONTINUOUS MODE OF OPERATION FOR LARGE VOLUME DOSING IN ANALYTICAL CARRIER AMPHOLYTE–FREE ISOELECTRIC FOCUSING OF PROTEINS APPLIED TO OFF–LINE DETECTION OF FRACTIONS

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