About: Electroosmotic Flow Changes Due to Interactions of Background Electrolyte with Polyethyleneimine Coating in Capillary Zone Electrophoresis of Proteins

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About: Electroosmotic Flow Changes Due to Interactions of Background Electrolyte with Polyethyleneimine Coating in Capillary Zone Electrophoresis of Proteins Goto Sponge NotDistinct Permalink

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Description	The properties and behavior of polyethyleneimine (PEI) covalently coated capillaries with respect to different background electrolytes used in capillary zone electrophoresis (CZE) is described. The coating stability and changes of inner surface charge in the capillary were followed by electroosmotic flow (EOF) measurement. The main interest was focused onto conjugate bases of carboxylic acids as anionic background electrolyte components (acetate, citrate, malate, malonate, tartrate and succinate). Interesting phenomenon in PEI coated capillaries was observed: the direction (and the magnitude) of EOF depends on the composition of the background electrolyte and at a certain pH it can reversibly change. To explain this behavior, a hypothesis of an ionic complex formation was suggested. With this knowledge, the PEI coated capillary was used for the separation of basic proteins in mentioned background electrolytes. The properties and behavior of polyethyleneimine (PEI) covalently coated capillaries with respect to different background electrolytes used in capillary zone electrophoresis (CZE) is described. The coating stability and changes of inner surface charge in the capillary were followed by electroosmotic flow (EOF) measurement. The main interest was focused onto conjugate bases of carboxylic acids as anionic background electrolyte components (acetate, citrate, malate, malonate, tartrate and succinate). Interesting phenomenon in PEI coated capillaries was observed: the direction (and the magnitude) of EOF depends on the composition of the background electrolyte and at a certain pH it can reversibly change. To explain this behavior, a hypothesis of an ionic complex formation was suggested. With this knowledge, the PEI coated capillary was used for the separation of basic proteins in mentioned background electrolytes. (en)
Title	Electroosmotic Flow Changes Due to Interactions of Background Electrolyte with Polyethyleneimine Coating in Capillary Zone Electrophoresis of Proteins Electroosmotic Flow Changes Due to Interactions of Background Electrolyte with Polyethyleneimine Coating in Capillary Zone Electrophoresis of Proteins (en)
skos:prefLabel	Electroosmotic Flow Changes Due to Interactions of Background Electrolyte with Polyethyleneimine Coating in Capillary Zone Electrophoresis of Proteins Electroosmotic Flow Changes Due to Interactions of Background Electrolyte with Polyethyleneimine Coating in Capillary Zone Electrophoresis of Proteins (en)
skos:notation	RIV/00216224:14310/06:00016303!RIV11-GA0-14310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA305/03/1100)
http://linked.open...iv/cisloPeriodika	14
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Havel, Josef Bittová, Miroslava Pazourek, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	473837
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/06:00016303
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	polyethyleneimine; capillary coating; electroosmotic flow; protein adsorption (en)
http://linked.open.../riv/klicoveSlovo	protein adsorption electroosmotic flow polyethyleneimine capillary coating
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[FA9C14966282]
http://linked.open...i/riv/nazevZdroje	Journal of Separation Science
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Synthesis and study of properties of neuroprotective peptide analogues derived of humanin
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	29
http://linked.open...iv/tvurceVysledku	Bittová, Miroslava Havel, Josef Pazourek, Jiří
issn	1615-9306
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Electroosmotic Flow Changes Due to Interactions of Background Electrolyte with Polyethyleneimine Coating in Capillary Zone Electrophoresis of Proteins

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