About: Isolation of polymerase chain reaction-ready bacterial DNA from Lake Baikal sediments by carboxyl-functionalised magnetic polymer microspheres

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Carboxyl group-containing magnetic nonporous poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) (P(HEMA-co-EDMA)) microspheres were used for the isolation of polymerase chain reaction(PCR)-ready DNA from samples of Baikal sediments. The occurence of false -negative results in PCR caused by the presence of extracelular inhibitors in DNA samples was solved using solid phase reversible DNA immobilisation. PCR-ready DNA was reversibly adsorbed to the microspheres in the presence of 8.0% PEG 6000 and 2.0 M sodium chloride concentrations. The adsorbed DNA was released from the microspheres in a low ioninic strength TE buffer. The quality of isolated DNA was checked by PCR amplification. Carboxyl group-containing magnetic nonporous poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) (P(HEMA-co-EDMA)) microspheres were used for the isolation of polymerase chain reaction(PCR)-ready DNA from samples of Baikal sediments. The occurence of false -negative results in PCR caused by the presence of extracelular inhibitors in DNA samples was solved using solid phase reversible DNA immobilisation. PCR-ready DNA was reversibly adsorbed to the microspheres in the presence of 8.0% PEG 6000 and 2.0 M sodium chloride concentrations. The adsorbed DNA was released from the microspheres in a low ioninic strength TE buffer. The quality of isolated DNA was checked by PCR amplification. (en)
Title	Isolation of polymerase chain reaction-ready bacterial DNA from Lake Baikal sediments by carboxyl-functionalised magnetic polymer microspheres Isolation of polymerase chain reaction-ready bacterial DNA from Lake Baikal sediments by carboxyl-functionalised magnetic polymer microspheres (en)
skos:prefLabel	Isolation of polymerase chain reaction-ready bacterial DNA from Lake Baikal sediments by carboxyl-functionalised magnetic polymer microspheres Isolation of polymerase chain reaction-ready bacterial DNA from Lake Baikal sediments by carboxyl-functionalised magnetic polymer microspheres (en)
skos:notation	RIV/00216224:14310/06:00016161!RIV10-GA0-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/05/2256), Z(AV0Z40500505)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Španová, Alena Rittich, Bohuslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	480520
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/06:00016161
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Magnetic microspheres; P(HEMA-co-EDMA); Lake Baikal sediments; Microbial DNA Magnetic separation; Polymerase chain reaction (PCR) (en)
http://linked.open.../riv/klicoveSlovo	Polymerase chain reaction (PCR) Lake Baikal sediments Microbial DNA Magnetic separation P(HEMA-co-EDMA) Magnetic microspheres
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[B30CA285D609]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Magnetic stimuli-responsive hydrophilic polymer microspheres for biomedicine/bioengineering
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	1130
http://linked.open...iv/tvurceVysledku	Horák, Daniel Rittich, Bohuslav Španová, Alena Štyriak, Igor Štyriaková, Iveta
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie
issn	0021-9673
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Isolation of polymerase chain reaction-ready bacterial DNA from Lake Baikal sediments by carboxyl-functionalised magnetic polymer microspheres Isolation of polymerase chain reaction-ready bacterial DNA from Lake Baikal sediments by carboxyl-functionalised magnetic polymer microspheres

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