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  • Biocompatibility of inner surface of capillaries in CE is critical for separation and detection of proteins and peptides. The problems come from adsorption of the peptides (proteins) on the surface because biomolecules are sensitive to changes in ionic strength, pH, and/or temperature of the electrolyte, etc. Therefore, modification of the fused silica capillary surface is often necessary. Procedures for capillary coating described in literature are usually complicated and time consuming. We optimized three types of relatively simple coating procedures suitable for protein and peptides separation: a/ polyethyleneimine (PEI), b/ 3-glycidoxypropyltrimethoxysilane (GPTMS), c/ 3-aminopropyltriethoxysilane (APS). Composition, concentration and pH of several background electrolyte were tested, namely citrate, phosphate, aminocaproate and TRIS. The capillary surface was characterized by determination of the electroosmotic flow (EOF).
  • Biocompatibility of inner surface of capillaries in CE is critical for separation and detection of proteins and peptides. The problems come from adsorption of the peptides (proteins) on the surface because biomolecules are sensitive to changes in ionic strength, pH, and/or temperature of the electrolyte, etc. Therefore, modification of the fused silica capillary surface is often necessary. Procedures for capillary coating described in literature are usually complicated and time consuming. We optimized three types of relatively simple coating procedures suitable for protein and peptides separation: a/ polyethyleneimine (PEI), b/ 3-glycidoxypropyltrimethoxysilane (GPTMS), c/ 3-aminopropyltriethoxysilane (APS). Composition, concentration and pH of several background electrolyte were tested, namely citrate, phosphate, aminocaproate and TRIS. The capillary surface was characterized by determination of the electroosmotic flow (EOF). (en)
Title
  • Capillary zone electrophoresis of proteins and neuroprotective peptides
  • Capillary zone electrophoresis of proteins and neuroprotective peptides (en)
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  • Capillary zone electrophoresis of proteins and neuroprotective peptides
  • Capillary zone electrophoresis of proteins and neuroprotective peptides (en)
skos:notation
  • RIV/00216224:14310/05:00025072!RIV10-GA0-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA305/03/1100)
http://linked.open...vai/riv/dodaniDat
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  • 514426
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  • RIV/00216224:14310/05:00025072
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  • capillary coating; neuroprotective peptides; capillary zone electrophoresis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [06CA4A0A2156]
http://linked.open...v/mistoKonaniAkce
  • Praha
http://linked.open...i/riv/mistoVydani
  • Geneva, Switzerland
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  • Third International and Twenty-Eighth European Peptide Symposium. Peptides 2004.
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  • Bittová, Miroslava
  • Havel, Josef
  • Pazourek, Jiří
  • Šulák, Ondřej
  • Gajdošová, Dagmar
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
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  • Kenes International
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  • 965-90833-0-0
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  • 14310
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