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  • The magnitude and the direction of electroosmotic flow (EOF) has fundamental influence to separation in capillary zone electrophoresis. Adsorption of proteins on the negative charged silica surface of uncoated capillaries can lead to analyte loss and to irreproducibility of analyses. Therefore, in biomolecules analyses, the inner surface modification of fused-silica capillaries is recommended. One of the used coating is polyethyleneimine (PEI): as a polycationic polymer it creates positively charged covalent coating on the capillary inner wall and hence minimizes the adsorption of basic proteins at acidic pH and maintains the electroosmotic flow stable [1]. In this work, the properties of PEI coated capillaries with respect to different background electrolytes are described. The changes of inner surface of the capillary were followed by electroosmotic flow measurement [2].
  • The magnitude and the direction of electroosmotic flow (EOF) has fundamental influence to separation in capillary zone electrophoresis. Adsorption of proteins on the negative charged silica surface of uncoated capillaries can lead to analyte loss and to irreproducibility of analyses. Therefore, in biomolecules analyses, the inner surface modification of fused-silica capillaries is recommended. One of the used coating is polyethyleneimine (PEI): as a polycationic polymer it creates positively charged covalent coating on the capillary inner wall and hence minimizes the adsorption of basic proteins at acidic pH and maintains the electroosmotic flow stable [1]. In this work, the properties of PEI coated capillaries with respect to different background electrolytes are described. The changes of inner surface of the capillary were followed by electroosmotic flow measurement [2]. (en)
Title
  • Studies of proteins adsorption in polyethyleneimine coated capillaries in capillary zone electrophoresis
  • Studies of proteins adsorption in polyethyleneimine coated capillaries in capillary zone electrophoresis (en)
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  • Studies of proteins adsorption in polyethyleneimine coated capillaries in capillary zone electrophoresis
  • Studies of proteins adsorption in polyethyleneimine coated capillaries in capillary zone electrophoresis (en)
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  • RIV/00216224:14310/05:00025070!RIV10-GA0-14310___
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  • P(GA305/03/1100)
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  • 545084
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  • RIV/00216224:14310/05:00025070
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  • capillary coating; polyethyleneimine; electroosmotic flow; protein adsorption (en)
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  • [531C928654DD]
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  • Pardubice
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  • 11th International Symposium on Separation Sciences ISSS 2005. Book of Abstracts.
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  • Bittová, Miroslava
  • Havel, Josef
  • Pazourek, Jiří
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  • Univerzita Pardubice
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  • 80-7194-771-7
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  • 14310
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