About: Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein

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About: Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A novel Arabidopsis thaliana mutant of one member of the PPR (pentatricopeptide repeat) gene family has been identified during the screening of 2500 Arabidopsis individual insertion T2 lines. The deviations in growth and development of the mutant plants appeared already at the initial growth. The germination was completely inhibited (about 30 to 40% of the T3 seeds in each line) or distinctly delayed in comparison with wt plants. The next development of the mutant seedlings varied further on agar medium. Complex phenotypic expression from post-embryonic lethality associated with abnormal pattern of cell division during globular to heart transition to fertile plants with just subtle phenotypic changes was observed within this individual mutant line. The Southern blot and segregation analysis revealed that the mutant plants contained one insert of T-DNA tightly linked to the mutant locus. A novel Arabidopsis thaliana mutant of one member of the PPR (pentatricopeptide repeat) gene family has been identified during the screening of 2500 Arabidopsis individual insertion T2 lines. The deviations in growth and development of the mutant plants appeared already at the initial growth. The germination was completely inhibited (about 30 to 40% of the T3 seeds in each line) or distinctly delayed in comparison with wt plants. The next development of the mutant seedlings varied further on agar medium. Complex phenotypic expression from post-embryonic lethality associated with abnormal pattern of cell division during globular to heart transition to fertile plants with just subtle phenotypic changes was observed within this individual mutant line. The Southern blot and segregation analysis revealed that the mutant plants contained one insert of T-DNA tightly linked to the mutant locus. (en)
Title	Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein (en)
skos:prefLabel	Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein (en)
skos:notation	RIV/00216224:14310/05:00021384!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Řepková, Jana
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	525740
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/05:00021384
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Arabidopsis thaliana; T-DNA; PPR; gene isolation (en)
http://linked.open.../riv/klicoveSlovo	Arabidopsis thaliana PPR gene isolation T-DNA
http://linked.open...ontrolniKodProRIV	[67A0F8041B4A]
http://linked.open...v/mistoKonaniAkce	České Budějovice
http://linked.open...i/riv/mistoVydani	České Budějovice
http://linked.open...i/riv/nazevZdroje	Book of Abstracts, 6th International Symposium in the Series Recent Advances in Plant Biotechnology, 12.-16.9.2005
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2005
http://linked.open...iv/tvurceVysledku	Hoyerová, K. Kocábek, Tomáš Řepková, Jana Vrba, L.
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2005-01-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Ústav molekulární biologie rostlin AV ČR
https://schema.org/isbn	80-86778-16-9
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein

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