About: Mycobacterial Haloalkane Dehalogenases: Cloning, Biochemical Properties and distribution.

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About: Mycobacterial Haloalkane Dehalogenases: Cloning, Biochemical Properties and distribution. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Haloalkane dehalogenases are enzymes catalysing the cleavage of the carbon-halogen bond by a hydrolytic mechanism. Genomes of Mycobacterium tuberculosis and M. bovis contain at least two open reading frames coding for the polypeptides showing a high sequence similarity with biochemically characterised haloalkane dehalogenases. In this paper we have described the cloning of the haloalkane dehalogenase genes dmbA and dmbB from M. bovis 5033/66 and proved the dehalogenase activity of their translation products. Both these genes are widely distributed among species of the M. tuberculosis complex, including M. bovis, M. bovis BCG, M. africanum, M. caprae, M. microti, and M. pinnipedii, as shown by the PCR screening of forty eight isolates from various hosts. DmbA and DmbB proteins were heterologously expressed in Escherichia coli and purified to homogeneity. The DmbB protein had to be expressed in a fusion with thioredoxin to obtain a soluble protein sample. Haloalkane dehalogenases are enzymes catalysing the cleavage of the carbon-halogen bond by a hydrolytic mechanism. Genomes of Mycobacterium tuberculosis and M. bovis contain at least two open reading frames coding for the polypeptides showing a high sequence similarity with biochemically characterised haloalkane dehalogenases. In this paper we have described the cloning of the haloalkane dehalogenase genes dmbA and dmbB from M. bovis 5033/66 and proved the dehalogenase activity of their translation products. Both these genes are widely distributed among species of the M. tuberculosis complex, including M. bovis, M. bovis BCG, M. africanum, M. caprae, M. microti, and M. pinnipedii, as shown by the PCR screening of forty eight isolates from various hosts. DmbA and DmbB proteins were heterologously expressed in Escherichia coli and purified to homogeneity. The DmbB protein had to be expressed in a fusion with thioredoxin to obtain a soluble protein sample. (en)
Title	Mycobacterial Haloalkane Dehalogenases: Cloning, Biochemical Properties and distribution. Mycobacterial Haloalkane Dehalogenases: Cloning, Biochemical Properties and distribution. (en)
skos:prefLabel	Mycobacterial Haloalkane Dehalogenases: Cloning, Biochemical Properties and distribution. Mycobacterial Haloalkane Dehalogenases: Cloning, Biochemical Properties and distribution. (en)
skos:notation	RIV/00216224:14310/05:00014724!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021622412)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Prokop, Zbyněk Fořtová, Andrea Pavlová, Martina Slaná, Iva Damborský, Jiří Chaloupková, Radka Monincová, Marta
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	531727
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/05:00014724
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	haloalkane dehalogenases; mycobacterium; cloning (en)
http://linked.open.../riv/klicoveSlovo	mycobacterium haloalkane dehalogenases cloning
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[8275ADA2624A]
http://linked.open...i/riv/nazevZdroje	Applied and Environmental Microbiology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	13 (xsd:int)
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	71
http://linked.open...iv/tvurceVysledku	Chaloupková, Radka Damborský, Jiří Fořtová, Andrea Nagata, Yuji Pavlík, Ivo Pavlová, Martina Prokop, Zbyněk Rychlík, Ivan Strouhal, Michal Monincová, Marta Bartoš, Milan Těšínská, Iva Mobius, Petra
http://linked.open...ain/vavai/riv/wos	000233225000035
http://linked.open...n/vavai/riv/zamer	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
issn	0099-2240
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Mycobacterial Haloalkane Dehalogenases: Cloning, Biochemical Properties and distribution.

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