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  • Staphylococcus xylosus and Staphylococcus equorum are two biochemically and phylogenetically close species. Athough S.xylosus is usually only transient on human and is primarily acquired from domestic animal and their products, it has been isolated also from human clinical specimens, mainly from urinary tract infection. Lack of the biochemical key tests makes differentiating of these species difficult. It is very important to find another reliable approach to distinguish S.xylosus and S.equorum species. A group of 18 presumptive S.xylosus strains from human clinical isolates were analysed by biochemical tests and ribotyping. SDS-PAGE protein analysis was performed to confirm the results of ribotyping. Biochemical properties were tested by API Staph and ID 32 STAPH commercial kits as well as by conventional tests. Ribotyping was done with EcoRI and HindIII restriction enzymes and a probe complementary to 16S-23S rRNA.
  • Staphylococcus xylosus and Staphylococcus equorum are two biochemically and phylogenetically close species. Athough S.xylosus is usually only transient on human and is primarily acquired from domestic animal and their products, it has been isolated also from human clinical specimens, mainly from urinary tract infection. Lack of the biochemical key tests makes differentiating of these species difficult. It is very important to find another reliable approach to distinguish S.xylosus and S.equorum species. A group of 18 presumptive S.xylosus strains from human clinical isolates were analysed by biochemical tests and ribotyping. SDS-PAGE protein analysis was performed to confirm the results of ribotyping. Biochemical properties were tested by API Staph and ID 32 STAPH commercial kits as well as by conventional tests. Ribotyping was done with EcoRI and HindIII restriction enzymes and a probe complementary to 16S-23S rRNA. (en)
Title
  • Characterization of presumptive Staphylococcus xylosus strains by SDS-PAGE protein profiles.
  • Characterization of presumptive Staphylococcus xylosus strains by SDS-PAGE protein profiles. (en)
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  • Characterization of presumptive Staphylococcus xylosus strains by SDS-PAGE protein profiles.
  • Characterization of presumptive Staphylococcus xylosus strains by SDS-PAGE protein profiles. (en)
skos:notation
  • RIV/00216224:14310/05:00012492!RIV10-GA0-14310___
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  • P(GA301/02/1505)
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  • 515029
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  • RIV/00216224:14310/05:00012492
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  • Staphylococcus xylosus; Staphylococcus equorum; SDS-PAGE; clinical specimen (en)
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  • [3A160C5D7FA4]
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  • Brno
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  • Brno
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  • IX. Pracovní setkání biochemiků a molekulárních biologů. Sborník příspěvků.
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  • Sedláček, Ivo
  • Petráš, Petr
  • Nováková, Dana
  • Štětina, Vlastimil
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  • Masarykova univerzita v Brně
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  • 80-210-3635-4
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  • 14310
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