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| rdf:type
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| Description
| - Protein p53 is a transcriptional factor, which is routinely attendant in a cell at low concentrations. Alzheimer's disease, Parkinson's disease, cystic fibrosis, prion diseases, and many types of cancer are considered to be protein p53 conformation diseases. Most of them are also known as amyloidogenic diseases due to the occurrence of pathological accumulation of insoluble aggregates with fibrillar conformation. Some neuroblastomas, carcinomas, and myelomas show an abnormal accumulation of the wild-type tumor suppressor protein p53 either in the cytoplasm or in the nucleus of the cell. Electrochemical methods could be a suitable tool for study of very low protein p53 intracellular concentrations. In our work, protein p53 was analyzed by flow injection analysis coupled with electrochemical detector. The optimized method was rapid (less then five minutes) and sensitive (protein p53 detection limit was 45 amol). The sensitive detection method was applied to the study structural changes of protein p53 (d
- Protein p53 is a transcriptional factor, which is routinely attendant in a cell at low concentrations. Alzheimer's disease, Parkinson's disease, cystic fibrosis, prion diseases, and many types of cancer are considered to be protein p53 conformation diseases. Most of them are also known as amyloidogenic diseases due to the occurrence of pathological accumulation of insoluble aggregates with fibrillar conformation. Some neuroblastomas, carcinomas, and myelomas show an abnormal accumulation of the wild-type tumor suppressor protein p53 either in the cytoplasm or in the nucleus of the cell. Electrochemical methods could be a suitable tool for study of very low protein p53 intracellular concentrations. In our work, protein p53 was analyzed by flow injection analysis coupled with electrochemical detector. The optimized method was rapid (less then five minutes) and sensitive (protein p53 detection limit was 45 amol). The sensitive detection method was applied to the study structural changes of protein p53 (d (en)
- Protein p53 je transkripční faktor, kerý se běžně vyskytuje v nízkých koncentracích. Alzheimerova, Parkinsonova nemoc, cystická fibróza, prionové nemoc a mnoho typů rakovin jsou označovány jako nemoci související se měnou konformace proteinu p53. Elektrochemické metody mohou být vhodným nástrojem pro studium velmi nízkých intracelulárních koncentrací proteinu p53. V naší práci byl protein p53 analyzován průtkovou injekční analýzou spojenou s elektrochemikcým detektorem. Optimalizovaná metoda byla rychlá méně než pět minut) a senzitivní (limit detekce proteinu p53 byl 45 amol). Optimalizovaná metoda byla dále použita pro studium strukturních změn proteinu p53 (denaturace a agregace). Byly pozorovány změny elektrocemických odpovědí individuálních proteinových forem. Popisovaná metoda přináší nový a efektivní nástroj pro studium běžných a nádorových buněk. (cs)
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| Title
| - Fast and sensitive electrochemical detection of native, denatured, and aggregated forms of tumor supressor protein p53
- Fast and sensitive electrochemical detection of native, denatured, and aggregated forms of tumor supressor protein p53 (en)
- Rychlá a sensitivní elektrochemická detekce nativní, denaturované a agregované formy nádorového supresoru proteinu p53 (cs)
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| skos:prefLabel
| - Fast and sensitive electrochemical detection of native, denatured, and aggregated forms of tumor supressor protein p53
- Fast and sensitive electrochemical detection of native, denatured, and aggregated forms of tumor supressor protein p53 (en)
- Rychlá a sensitivní elektrochemická detekce nativní, denaturované a agregované formy nádorového supresoru proteinu p53 (cs)
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| skos:notation
| - RIV/00216224:14310/04:00011033!RIV09-GA0-14310___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GP525/04/P132), P(LN00A081)
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/04:00011033
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - protein p53; electrochemical detection; structural forms; aggregation; denaturation (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/mistoVydani
| - Bethesda, Maryland 20814-2762
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| http://linked.open...i/riv/nazevZdroje
| - Molecular Biology of the Cell
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Adam, Vojtěch
- Kizek, René
- Masařík, Michal
- Průša, Richard
- Jelen, František
- Potěšil, David
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open.../riv/zahajeniAkce
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| issn
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| number of pages
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| http://purl.org/ne...btex#hasPublisher
| - The American Society for Cell Biology
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| http://localhost/t...ganizacniJednotka
| |
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