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| rdf:type
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| Description
| - Paracoccus denitrificans is a non-fermentative, facultatively autotrophic soil bacterium often studied in the field of bioenergetics, particularly due to resemblance of its aerobic respiratory chain to that of mitochondria. Also an aspect of a great nutritional adaptability was discovered, related to the ability of exploiting various electron donors and electron acceptors for maintenance and growth. To discuss mechanisms underlying regulation of gene expression by growth conditions, a high-throughput proteome mapping is one of the most effective tools to-date. For 2-D electrophoretic analysis of whole-cell extract and its membrane fraction, isoelectric focusation with immobilized pH gradients in the first dimension and SDS-PAGE in the second dimension were used. Using this approach, more than 800 protein spots of total cell extract and membrane fraction were detected in the range pI 3-10 and Mr 15-100 kDa. We compared complex protein composition of whole cells of P. denitrificans cultivated under aero
- Paracoccus denitrificans is a non-fermentative, facultatively autotrophic soil bacterium often studied in the field of bioenergetics, particularly due to resemblance of its aerobic respiratory chain to that of mitochondria. Also an aspect of a great nutritional adaptability was discovered, related to the ability of exploiting various electron donors and electron acceptors for maintenance and growth. To discuss mechanisms underlying regulation of gene expression by growth conditions, a high-throughput proteome mapping is one of the most effective tools to-date. For 2-D electrophoretic analysis of whole-cell extract and its membrane fraction, isoelectric focusation with immobilized pH gradients in the first dimension and SDS-PAGE in the second dimension were used. Using this approach, more than 800 protein spots of total cell extract and membrane fraction were detected in the range pI 3-10 and Mr 15-100 kDa. We compared complex protein composition of whole cells of P. denitrificans cultivated under aero (en)
- Proteomová analýza bakterie Paracoccus denitrificans v závislosti na použitém terminálním akceptoru elektronů a přítomnosti azidu. Jedná se o předběžnou prezentaci proteomové studie, která byla následně publikována: Bouchal P., Přecechtělová P., Zdráhal Z., Kučera I.: Protein composition of Paracoccus denitrificans cells grown on various electron acceptors and in the presence of azide. Proteomics 2004, 4, 2662-2671. (cs)
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| Title
| - The effects of terminal electron acceptor and sodium azide on the Paracoccus denitrificans protein composition
- The effects of terminal electron acceptor and sodium azide on the Paracoccus denitrificans protein composition (en)
- Vliv terminálního akceptoru elektronů a azidu na proteinové složení bakterie Paracoccus denitrificans (cs)
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| skos:prefLabel
| - The effects of terminal electron acceptor and sodium azide on the Paracoccus denitrificans protein composition
- The effects of terminal electron acceptor and sodium azide on the Paracoccus denitrificans protein composition (en)
- Vliv terminálního akceptoru elektronů a azidu na proteinové složení bakterie Paracoccus denitrificans (cs)
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| skos:notation
| - RIV/00216224:14310/04:00010926!RIV08-MSM-14310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA203/01/1589), Z(MSM 143100005), Z(MSM 143100008)
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/04:00010926
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Paracoccus denitrificans; proteome analysis; terminal electron acceptor; azide (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - Acta Universitatis Palackianae Olomucensis - Chemica 43S
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Zdráhal, Zbyněk
- Bouchal, Pavel
- Kučera, Igor
- Přecechtělová, Petra
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open.../riv/zahajeniAkce
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| http://linked.open...n/vavai/riv/zamer
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| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| - Univerzita Palackého v Olomouci
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| https://schema.org/isbn
| |
| http://localhost/t...ganizacniJednotka
| |
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