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  • In our case study, we measured the functional diversity of the microbial communities of twelve soils from the small natural area to assess if this assay is suitable for routine monitoring of soil biological quality. We found the BIOLOG assay meets especially practical benefits in routine monitoring of soils being simple and quick assay. However, we confirmed the ambiguity about the most appropriate analysis of the BIOLOG multivariate data and about the best parameter, which can be derived from the assay. The different analyses of the data were examined and various parameters derived from the BIOLOG assay were comparatively used to contribute to the discussion of how the data should be evaluated. We showed that not-normalized raw absorbances or trapezoid areas should be used for calculation of diversity index if the inoculum was standardized. There was no single answer to what parameter provided more correct results in the multivariate statistical analysis. Evaluating at least one not-normalized (e.g.
  • In our case study, we measured the functional diversity of the microbial communities of twelve soils from the small natural area to assess if this assay is suitable for routine monitoring of soil biological quality. We found the BIOLOG assay meets especially practical benefits in routine monitoring of soils being simple and quick assay. However, we confirmed the ambiguity about the most appropriate analysis of the BIOLOG multivariate data and about the best parameter, which can be derived from the assay. The different analyses of the data were examined and various parameters derived from the BIOLOG assay were comparatively used to contribute to the discussion of how the data should be evaluated. We showed that not-normalized raw absorbances or trapezoid areas should be used for calculation of diversity index if the inoculum was standardized. There was no single answer to what parameter provided more correct results in the multivariate statistical analysis. Evaluating at least one not-normalized (e.g. (en)
  • V této případové studii jsme měřili funkční diverzitu půdních mikrobiálních společenstev dvanácti půd z malé přírodní oblasti s cílem posoudit vhodnost použití tohoto přístupu pro rutinní monitoring půdní biologické kvality. Shledali jsme, že metoda BIOLOG splňuje zejména praktické výhody pro rutinní monitoring půd, protože je rychlá a jednoduchá. Na druhou stranu jsme potvrdili nejednoznačnost kolem nejvhodnější analýzy vícerozměrných dat z použité metody. Také výběr nejlepšího parametru metody je problematický. Abychom přispěli k diskusi, jak mají být data z metody BIOLOG hodnocena, použili jsme pro srovnání různé parametry a způsoby jejich vyhodnocení. Ukázalo se, že pokud bylo inokulum standardizováno, měly by být pro výpočet indexu diverzity použity nenormalizované absorbance či trapezoid areas. Co se týká vícerozměrných statistických analýz, nelze jednoznačně určit, který parametr je korektnější. Bylo doporučeno hodnocení alespoň jednoho nenormalizovaného (např. trapezoid area) a jednoho normali (cs)
Title
  • Hodnocení funkční diverzity půdních mikrobiálních společenstev - případová studie (cs)
  • Evaluation of functional diversity of soil microbial communities - a case study
  • Evaluation of functional diversity of soil microbial communities - a case study (en)
skos:prefLabel
  • Hodnocení funkční diverzity půdních mikrobiálních společenstev - případová studie (cs)
  • Evaluation of functional diversity of soil microbial communities - a case study
  • Evaluation of functional diversity of soil microbial communities - a case study (en)
skos:notation
  • RIV/00216224:14310/04:00010825!RIV08-MSM-14310___
http://linked.open.../vavai/riv/strany
  • 141-148
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA525/03/0367), P(SI/340/1/00), Z(MSM 141100003)
http://linked.open...iv/cisloPeriodika
  • 4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 563357
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/04:00010825
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • BIOLOG; soil microorganisms; soil monitoring; functional diversity (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [BDD1A76663AA]
http://linked.open...i/riv/nazevZdroje
  • Plant Soil and Environment
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 50
http://linked.open...iv/tvurceVysledku
  • Hofman, Jakub
  • Holoubek, Ivan
  • Švihálek, Jiří
http://linked.open...n/vavai/riv/zamer
issn
  • 0370-663X
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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