| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Bakteriální druh Staphylococcus aureus produkuje exfoliativní toxiny, které způsobují u lidí a zejména u kojenců kožní puchýřnaté onemocnění, jehož nejtěžší formou je syndrom opařené kůže. Gen eta kódující toxin ETA se nachází v DNA profága, což je genom mírného fága začleněný do chromozomu hostitelské bakterie. Mírné stafylofágy lyzogenní konverzí mění genetické a fenotypové vlastnosti hostitelského kmene. V této práci byly izolovány bakteriofágy z osmi ETA-pozitivních kmenů S. aureus. Metodou multiplex PCR bylo pět fágů zařazeno do serologické skupiny A a tři fágy do serologické skupiny B. PCR detekcí byl u nich prokázán strukturní gen eta. Všechny fágy byly charakterizovány restrikční analýzou DNA pomocí restriktázy HindIII takto: ve skupině A-fágů byly rozlišeny tři subtypy A-1, A-2 a A-3, zatímco u B-fágů byly rozlišeny dva subtypy, B-1a B-2. Tři fágy byly použity k přenosu genu eta do recipietního kmene. Jeden A-fág a dva B-fágy lyzogenizovaly ETA-negativní kmen S. aureus. Bylo zjištěno, že gen (cs)
- Two exfoliative toxins, ETA and ETB, produced by certain strains of S. aureus are the major causative agents of blistering skin disorders with the most severe form the Staphylococcal Scalded Skin Syndrome, primarily affecting infants and young children. The eta gene encoding ETA is located on the chromosome, whereas the etb gene encoding ETB is located on a large plasmid. Temperate bacteriophages carrying the eta gene and converting exfoliative toxin A production in S. aureus, have been recently, found. Eight bacteriophages isolated from 8 human ETA-positive strains of S. aureus by UV induction were classified into serogroups, screened for carriage of the eta gene by PCR, and characterized by HindIII restriction analysis. Three of them were used for the ETA conversion experiments. Location of prophages in lysogens was revealed by PFGE and ETA production was detected by RPLA. Five of the phages were classified into serogroup A (A-phages) and three into serogroup B (B-phages). Restriction enzyme HindIII
- Two exfoliative toxins, ETA and ETB, produced by certain strains of S. aureus are the major causative agents of blistering skin disorders with the most severe form the Staphylococcal Scalded Skin Syndrome, primarily affecting infants and young children. The eta gene encoding ETA is located on the chromosome, whereas the etb gene encoding ETB is located on a large plasmid. Temperate bacteriophages carrying the eta gene and converting exfoliative toxin A production in S. aureus, have been recently, found. Eight bacteriophages isolated from 8 human ETA-positive strains of S. aureus by UV induction were classified into serogroups, screened for carriage of the eta gene by PCR, and characterized by HindIII restriction analysis. Three of them were used for the ETA conversion experiments. Location of prophages in lysogens was revealed by PFGE and ETA production was detected by RPLA. Five of the phages were classified into serogroup A (A-phages) and three into serogroup B (B-phages). Restriction enzyme HindIII (en)
|
| Title
| - Characterization of resident bacteriophages and their role in conversion of exfoliative toxin A production in <I>Staphylococcus aureus</I>
- Characterization of resident bacteriophages and their role in conversion of exfoliative toxin A production in <I>Staphylococcus aureus</I> (en)
- Charakterizace rezidentních bakteriofágů a jejich role v konverzi exfoliatin A produkujících kmenů Staphylococcus aureus (cs)
|
| skos:prefLabel
| - Characterization of resident bacteriophages and their role in conversion of exfoliative toxin A production in <I>Staphylococcus aureus</I>
- Characterization of resident bacteriophages and their role in conversion of exfoliative toxin A production in <I>Staphylococcus aureus</I> (en)
- Charakterizace rezidentních bakteriofágů a jejich role v konverzi exfoliatin A produkujících kmenů Staphylococcus aureus (cs)
|
| skos:notation
| - RIV/00216224:14310/04:00010415!RIV09-GA0-14310___
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA301/02/1505), Z(MSM 143100008)
|
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/04:00010415
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - staphylococcus; exfoliative toxin A; bacteriophage; lysogenic conversion (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...v/mistoKonaniAkce
| - Charleston, South Carolina, USA
|
| http://linked.open...i/riv/mistoVydani
| - Charleston, South Carolina, USA
|
| http://linked.open...i/riv/nazevZdroje
| - 11th International Symposium on Staphylococci & Staphylococcal Infections. Plenary Summaries & Poster Abstracts
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Doškař, Jiří
- Pantůček, Roman
- Růžičková, Vladislava
- Petráš, Petr
- Pekarová, Mária
|
| http://linked.open...vavai/riv/typAkce
| |
| http://linked.open.../riv/zahajeniAkce
| |
| http://linked.open...n/vavai/riv/zamer
| |
| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| - Medical University of South Carolina
|
| http://localhost/t...ganizacniJednotka
| |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)