About: Piezoelectric biosensors for real-time monitoring of hybridization and detection of the hepatitis C virus     Goto   Sponge   NotDistinct   Permalink

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Description
  • Piezoelektrický krystalový resonátor byl modifikován oligonukleotidovou probou a použit pro detekci virové nukleové kyseliny v seru. Biotinylované proby byly navázany na monovrstvu avidinu kovalentně vázanou na povrch zlatých elektrod. Virová RNA byla pomocí RT-PCR techniky převedena na DNA, které pak byly detekovány PZ biosensorem. Pro srovnání byly vzorky analyzovány také soupravou Amplicor firmy Roche (fotometrické stanovení na mikrodestičce). (cs)
  • The piezoelectric quartz crystal resonators modified with oligonucleotide probes were used for detection of hepatitis C virus in serum. The gold electrodes on either rough or smooth surface crystals were modified with a self-assembled monolayer of cystamine. After activation with glutaraldehyde, either avidin or streptavidin were immobilized and used for attachment of biotinylated DNA probes (4 different sequences). Thus prepared piezoelectric biosensors were used in a flow-through setup for direct monitoring of DNA resulting from the reverse transcriptase linked polymerase chain reaction (RT-PCR) amplification of the original viral RNA. The samples of patients with hepatitis C were analyzed and the results were compared with the standard RT-PCR procedure (Amplicor test kit of Roche, microwell format with spectrophotometric evaluation). The piezoelectric hybridization assay was completed in 10 min and the same sensing surface was suitable for repeated use.
  • The piezoelectric quartz crystal resonators modified with oligonucleotide probes were used for detection of hepatitis C virus in serum. The gold electrodes on either rough or smooth surface crystals were modified with a self-assembled monolayer of cystamine. After activation with glutaraldehyde, either avidin or streptavidin were immobilized and used for attachment of biotinylated DNA probes (4 different sequences). Thus prepared piezoelectric biosensors were used in a flow-through setup for direct monitoring of DNA resulting from the reverse transcriptase linked polymerase chain reaction (RT-PCR) amplification of the original viral RNA. The samples of patients with hepatitis C were analyzed and the results were compared with the standard RT-PCR procedure (Amplicor test kit of Roche, microwell format with spectrophotometric evaluation). The piezoelectric hybridization assay was completed in 10 min and the same sensing surface was suitable for repeated use. (en)
Title
  • Piezoelektrický biosensor pro sledování hybridizace v reálném čase a pro stanovení viru hepatitidy C (cs)
  • Piezoelectric biosensors for real-time monitoring of hybridization and detection of the hepatitis C virus
  • Piezoelectric biosensors for real-time monitoring of hybridization and detection of the hepatitis C virus (en)
skos:prefLabel
  • Piezoelektrický biosensor pro sledování hybridizace v reálném čase a pro stanovení viru hepatitidy C (cs)
  • Piezoelectric biosensors for real-time monitoring of hybridization and detection of the hepatitis C virus
  • Piezoelectric biosensors for real-time monitoring of hybridization and detection of the hepatitis C virus (en)
skos:notation
  • RIV/00216224:14310/04:00010106!RIV08-MSM-14310___
http://linked.open.../vavai/riv/strany
  • 145-151
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(OC 518.30)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 579573
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/04:00010106
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Piezoelectric biosesnsor; DNA; Hepatitis C; PCR; Hybridization (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [198D3E1D6C33]
http://linked.open...i/riv/nazevZdroje
  • Journal of Virological Methods
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 117
http://linked.open...iv/tvurceVysledku
  • Skládal, Petr
  • Yamanaka, Hideko
  • Costa, Paolo
  • Santos Ricardi, Carla
issn
  • 0166-0934
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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