About: Expression and purification of putative mycobacterial glycosyltransferase Rv3782 for crystallographic studies     Goto   Sponge   NotDistinct   Permalink

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Description
  • A putative rhamnosyltransferase Rv3782 belongs to a group of glycosyltansferases (GTs) that are responsible for biosynthesis of cell wall envelope of Mycobacterium tuberculosis. Mycobacterial cell wall contains some unique sugar components that play a crucial role in host-pathogen interaction and is responsible for high pathogen resistance against common therapeutic agents. Thus these GTs are interesting as potential drug targets. In previous part of our research we selected some genes of putative GTs (using various bioinformatics tools applied to M. tuberculosis H37Rv genome sequence) that should be mostly of interest. At present study we have been focused on structural analysis of Rv3782. The main problem is to obtain a sufficient amount of soluble and pure protein for crystallization studies. Most of the expressed protein forms inclusion bodies, probably because of its toxicity for host cells, and there are additional losses caused by high GTs hydrofobicity that complicates its purification.
  • A putative rhamnosyltransferase Rv3782 belongs to a group of glycosyltansferases (GTs) that are responsible for biosynthesis of cell wall envelope of Mycobacterium tuberculosis. Mycobacterial cell wall contains some unique sugar components that play a crucial role in host-pathogen interaction and is responsible for high pathogen resistance against common therapeutic agents. Thus these GTs are interesting as potential drug targets. In previous part of our research we selected some genes of putative GTs (using various bioinformatics tools applied to M. tuberculosis H37Rv genome sequence) that should be mostly of interest. At present study we have been focused on structural analysis of Rv3782. The main problem is to obtain a sufficient amount of soluble and pure protein for crystallization studies. Most of the expressed protein forms inclusion bodies, probably because of its toxicity for host cells, and there are additional losses caused by high GTs hydrofobicity that complicates its purification. (en)
  • A putative rhamnosyltransferase Rv3782 belongs to a group of glycosyltansferases (GTs) that are responsible for biosynthesis of cell wall envelope of Mycobacterium tuberculosis. Mycobacterial cell wall contains some unique sugar components that play a crucial role in host-pathogen interaction and is responsible for high pathogen resistance against common therapeutic agents. Thus these GTs are interesting as potential drug targets. In previous part of our research we selected some genes of putative GTs (using various bioinformatics tools applied to M. tuberculosis H37Rv genome sequence) that should be mostly of interest. At present study we have been focused on structural analysis of Rv3782. The main problem is to obtain a sufficient amount of soluble and pure protein for crystallization studies. Most of the expressed protein forms inclusion bodies, probably because of its toxicity for host cells, and there are additional losses caused by high GTs hydrofobicity that complicates its purification. (cs)
Title
  • Expression and purification of putative mycobacterial glycosyltransferase Rv3782 for crystallographic studies
  • Expression and purification of putative mycobacterial glycosyltransferase Rv3782 for crystallographic studies (en)
  • Expression and purification of putative mycobacterial glycosyltransferase Rv3782 for crystallographic studies (cs)
skos:prefLabel
  • Expression and purification of putative mycobacterial glycosyltransferase Rv3782 for crystallographic studies
  • Expression and purification of putative mycobacterial glycosyltransferase Rv3782 for crystallographic studies (en)
  • Expression and purification of putative mycobacterial glycosyltransferase Rv3782 for crystallographic studies (cs)
skos:notation
  • RIV/00216224:14310/03:00009308!RIV08-MSM-14310___
http://linked.open.../vavai/riv/strany
  • 26
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM 143100005)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...dnocenehoVysledku
  • 606905
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/03:00009308
http://linked.open...riv/jazykVysledku
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  • mycobacterium tuberculosis; glycosyltransferase (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [C6C767EAA06C]
http://linked.open...v/mistoKonaniAkce
  • Brno
http://linked.open...i/riv/mistoVydani
  • Brno
http://linked.open...i/riv/nazevZdroje
  • VII. pracovní setkání biochemiků a molekulárních biologů
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Imberty, Anne
  • Kostlánová, Nikola
  • Wimmerová, Michaela
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
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  • Masarykova univerzita
https://schema.org/isbn
  • 80-210-3053-4
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  • 14310
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